Hide Mallorie auteur aut IRD Singh R. auteur aut IRD Kumar B. auteur aut IRD Banuls Anne-Laure auteur aut IRD Sundar S. auteur aut IRD text journalArticle eng text/pdf born digital access Current procedures for diagnosing Leishmania parasites from patients involve invasive and dangerous tissue aspiration. We have developed a non-invasive and highly sensitive microculture method that can isolate parasites from the buffy coat of the patient's peripheral blood. The parasites were cultured in 96-well culture plates. Nineteen parasitologically proven visceral leishmaniasis (VL) patients were included in the study. Using this technique, we were able to isolate parasites from 16 (84%) samples. However, all 19 (100%) samples were positive on culture of splenic aspirates. We conclude that this technique is useful for the isolation and cryoconservation of parasites from patients' blood. This simple method could be tried as a first-instance alternative before other more sensitive procedures such as splenic aspirate; however, negative results should be confirmed by tests with higher sensitivity. specialized Leishmania parasite isolation patient peripheral blood buffy coat non invasive method 052 102 3 197-200 2007 0001-706X https://www.documentation.ird.fr/hor/fdi:010040713 10.1016/j.actatropica.2007.04.015 0001-706X [F B010040713] https://www.documentation.ird.fr/hor/fdi:010040713 https://www.documentation.ird.fr/intranet/publi/2007/10/010040713.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2007-11-06 2017-08-23 fdi:010040713 fre