@article{fdi:010040713,
  title = {{A} microculture technique for isolating live {L}eishmania parasites from peripheral blood of visceral leishmaniasis patients},
  author = {{H}ide, {M}allorie and {S}ingh, {R}. and {K}umar, {B}. and {B}anuls, {A}nne-{L}aure and {S}undar, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}urrent procedures for diagnosing {L}eishmania parasites from patients involve invasive and dangerous tissue aspiration. {W}e have developed a non-invasive and highly sensitive microculture method that can isolate parasites from the buffy coat of the patient's peripheral blood. {T}he parasites were cultured in 96-well culture plates. {N}ineteen parasitologically proven visceral leishmaniasis ({VL}) patients were included in the study. {U}sing this technique, we were able to isolate parasites from 16 (84%) samples. {H}owever, all 19 (100%) samples were positive on culture of splenic aspirates. {W}e conclude that this technique is useful for the isolation and cryoconservation of parasites from patients' blood. {T}his simple method could be tried as a first-instance alternative before other more sensitive procedures such as splenic aspirate; however, negative results should be confirmed by tests with higher sensitivity.},
  keywords = {{L}eishmania ; parasite isolation ; patient peripheral blood ; buffy coat ; non invasive method},
  booktitle = {},
  journal = {{A}cta {T}ropica},
  volume = {102},
  numero = {3},
  pages = {197--200},
  ISSN = {0001-706{X}},
  year = {2007},
  DOI = {10.1016/j.actatropica.2007.04.015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040713},
}