@article{fdi:010040686,
  title = {{I}mpact of {HIV}-1 genetic diversity on plasma {HIV}-1 {RNA} quantification - {U}sefulness of the {A}gence {N}ationale de {R}echerches sur le {SIDA} second-generation long terminal repeat-based real-time reverse transcriptase polymerase chain reaction test},
  author = {{R}ouet, {F}. and {C}haix, {M}. {L}. and {N}errienet, {E}. and {N}go {G}iang {H}uong, {N}. and {P}lantier, {D}. {C}. and {B}urgard, {M}. and {P}eeters, {M}artine and {D}amond, {F}. and {E}kouevi, {D}. {K}. and {M}sellati, {P}hilippe and {F}erradini, {L}. and {R}ukobo, {S}. and {M}arechal, {V}. and {S}chvachsa, {N}. and {W}akrim, {L}. and {R}afalimanana, {C}. and {R}akotoambinina, {B}. and {V}iard, {J}. {P}. and {S}eigneurin, {J}. {M}. and {R}ouzioux, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he high genetic diversity of {HIV}-1 has a major impact on the quantification of plasma {HIV}-1 {RNA}, representing an increasingly difficult challenge. {A} total of 898 plasma specimens positive for {HIV}-1 {RNA} by commercial assays ({A}mplicor v1.5; {R}oche {D}iagnostic {S}ystems, {A}lameda, {CA} or {V}ersant v3.0; {B}ayer {D}iagnostics, {E}meryville, {CA}) were tested using the {A}gence {N}ationale de {R}echerches sur le {SIDA} second-generation ({G}2) real-time reverse transcriptase polymerase chain reaction ({RT}-{PCR}) test: 518 samples containing {HIV}-1 of known subtype, including 88 from 2 subtype panels and 430 harboring {B} (n = 266) and non-{B} (n = 164) group {M} {HIV}-1 subtypes from patients followed up in 2002 through 2005 at {N}ecker {H}ospital ({P}aris, {F}rance), and 380 samples from 10 different countries ({A}rgentina, {C}ambodia, {C}ameroon, {C}entral {A}frican {R}epublic, {F}rance, {I}vory {C}oast, {M}adagascar, {M}orocco, {T}hailand, and {Z}imbabwe). {HFV}-1 {RNA} values obtained by {G}2 real-time {PCR} were highly correlated with those obtained by the {A}mplicor v1.5 for {B} and non-{B} subtypes ({R}-2 = 0.892 and 0.892, respectively) and for samples from diverse countries ({R}-2 = 0.867 and 0.893 for real-time {PCR} vs. {A}mplicor v1.5 and real-time {PCR} vs. {V}ersant v3.0, respectively). {A}pproximately 30% of specimens harboring non-{B} subtypes were underquantified by at least -0.51 log(10) in {A}mplicor v1.5 versus 5% underquantified in {G}2 real-time {PCR}. {D}iscrepant results were also obtained with subtype {B} samples (14% underquantified by {A}mplicor v1.5 vs. 7% by {G}2 real-time {PCR}). {S}imilar percentages were observed when comparing results obtained with the {G}2 real-time {PCR} assay with those obtained using the {V}ersant assay. {A}ddressing {HIV}-1 diversity, continual monitoring of {HIV}-1 {RNA} assays, together with molecular epidemiology studies, is required to improve the accuracy of all {HIV} {RNA} assays.},
  keywords = {{HIV} 1 diversity ; {HIV} 1 {RNA} viral load ; real time polymerase chain reaction},
  booktitle = {},
  journal = {{J}aids {J}ournal of {A}cquired {I}mmune {D}eficiency {S}yndromes},
  volume = {45},
  numero = {4},
  pages = {380--388},
  ISSN = {1525-4135},
  year = {2007},
  DOI = {10.1097/{QAI}.0b013e3180640cf5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040686},
}