%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Petitjean, G.
%A Becquart, Pierre
%A Tuaillon, E.
%A Al Tabaa, Y.
%A Valea, D.
%A Huguet, M. F.
%A Meda, N.
%A Van de Perre, P.
%A Vendrell, J. P.
%T Isolation and characterization of HIV-1-infected resting CD4(+) T lymphocytes in breast milk
%D 2007
%L fdi:010040684
%G ENG
%J Journal of Clinical Virology
%@ 1386-6532
%K breast milk cells ; CD4+ T lymphocytes isolation ; ELISpot assay ; flow cytometry ; HIV 1 reservoir
%M CC:0002486313-0001
%N 1
%P 1-8
%R 10.1016/j.jcv.2007.02.004
%U https://www.documentation.ird.fr/hor/fdi:010040684
%> https://www.documentation.ird.fr/intranet/publi/2007/09/010040684.pdf
%V 39
%W Horizon (IRD)
%X An HIV-1 reservoir comprised primarily of latently infected resting CD4(+) T lymphocytes that can be stimulated in vivo to produce virus may play a critical role in mother-to-child postnatal transmission of HIV-1 by breastfeeding. Here, we describe an experimental protocol for the detection of resting CD4(+) T cell HIV-1 reservoir from breast milk. We adapted a method for the purification of resting CD4(+) T lymphocytes in blood to isolate resting CD4 T+ cells in breast milk from HIV-1-infected-lactating women (n = 18) and from controls (n = 3). Purified resting CD4 T+ cells from blood and breast milk samples of HIV-1-infected-lactating women were polyclonally stimulated to characterize and enumerate HIV-1-antigen-secreting cells (HIV-1-Ag-SCs) by an enzyme-linked immunospot (ELISpot) assay. Resting CD4 T+ cells represented more than 90% of purified viable breast milk cells. CD4(+) T cell polyclonal stimulation combined with the ELISpot assay led to the characterization of a breast milk T cell HIV-1 reservoir greater than the blood reservoir (median 400 and 57.14 HIV-1-Ag-SCs/10(6) resting CD4(+) T cells, respectively,p < 0.001). our strategy could be adapted to other body fluids and be useful for characterizing new HIV-1 reservoirs.
%$ 052