@article{fdi:010040684,
  title = {{I}solation and characterization of {HIV}-1-infected resting {CD}4(+) {T} lymphocytes in breast milk},
  author = {{P}etitjean, {G}. and {B}ecquart, {P}ierre and {T}uaillon, {E}. and {A}l {T}abaa, {Y}. and {V}alea, {D}. and {H}uguet, {M}. {F}. and {M}eda, {N}. and {V}an de {P}erre, {P}. and {V}endrell, {J}. {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}n {HIV}-1 reservoir comprised primarily of latently infected resting {CD}4(+) {T} lymphocytes that can be stimulated in vivo to produce virus may play a critical role in mother-to-child postnatal transmission of {HIV}-1 by breastfeeding. {H}ere, we describe an experimental protocol for the detection of resting {CD}4(+) {T} cell {HIV}-1 reservoir from breast milk. {W}e adapted a method for the purification of resting {CD}4(+) {T} lymphocytes in blood to isolate resting {CD}4 {T}+ cells in breast milk from {HIV}-1-infected-lactating women (n = 18) and from controls (n = 3). {P}urified resting {CD}4 {T}+ cells from blood and breast milk samples of {HIV}-1-infected-lactating women were polyclonally stimulated to characterize and enumerate {HIV}-1-antigen-secreting cells ({HIV}-1-{A}g-{SC}s) by an enzyme-linked immunospot ({ELIS}pot) assay. {R}esting {CD}4 {T}+ cells represented more than 90% of purified viable breast milk cells. {CD}4(+) {T} cell polyclonal stimulation combined with the {ELIS}pot assay led to the characterization of a breast milk {T} cell {HIV}-1 reservoir greater than the blood reservoir (median 400 and 57.14 {HIV}-1-{A}g-{SC}s/10(6) resting {CD}4(+) {T} cells, respectively,p < 0.001). our strategy could be adapted to other body fluids and be useful for characterizing new {HIV}-1 reservoirs.},
  keywords = {breast milk cells ; {CD}4+ {T} lymphocytes isolation ; {ELIS}pot assay ; flow cytometry ; {HIV} 1 reservoir},
  booktitle = {},
  journal = {{J}ournal of {C}linical {V}irology},
  volume = {39},
  numero = {1},
  pages = {1--8},
  ISSN = {1386-6532},
  year = {2007},
  DOI = {10.1016/j.jcv.2007.02.004},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040684},
}