@article{fdi:010040672,
  title = {{A} non-radiolabeled heme-{GSH} interaction test for the screening of antimalarial compounds},
  author = {{G}aravito, {G}. and {M}onje, {M}. {C}. and {M}aurel, {S}. and {V}alentin, {A}. and {N}epveu, {F}ran{\c{c}}oise and {D}eharo, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{I}ntraerythrocytic {P}lasmodium produces large amounts of toxic heme during the digestion of hemoglobin, a parasite specific pathway. {H}eme is then partially biocristallized into hemozin and mostly detoxified by reduced glutathione. {W}e proposed an in vitro micro assay to test the ability of drugs to inhibit heme-glutathione dependent degradation. {A}s glutathione and o-phthalaldehyde form a fluorescent adduct, we followed the extinction of the fluorescent signal when heme was added with or without antimalarial compounds. {I}n this assay, 50 mu {M} of amodiaquine, arthemether, chloroquine, methylene blue, mefloquine and quinine inhibited the interaction between glutathione (50 mu {M}) and heme (50 mu {M}), while atovaquone did not. {C}onsequently, this test could detect drugs that can inhibit heme-{GSH} degradation in a fast, simple and specific way, making it suitable for high throughput screening of potential antimalarials.},
  keywords = {{P}lasmodium falciparum ; glutathione ; heme ; antimalarials ; screening ; {GSH} ; reduced glutathione},
  booktitle = {},
  journal = {{E}xperimental {P}arasitology},
  volume = {116},
  numero = {3},
  pages = {311--313},
  ISSN = {0014-4894},
  year = {2007},
  DOI = {10.1016/j.exppara.2007.01.005},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040672},
}