%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Virreira, M.
%A Truyens, C.
%A Alonso Vega, C.
%A Brutus, Laurent
%A Jijena, J.
%A Torrico, F.
%A Carlier, Yves
%A Svoboda, M.
%T Comparison of Trypanosoma cruzi lineages and levels of parasitic DNA in infected mothers and their newborns
%D 2007
%L fdi:010040662
%G ENG
%J American Journal of Tropical Medicine and Hygiene
%@ 0002-9637
%M CC:0002479798-0017
%N 1
%P 102-106
%U https://www.documentation.ird.fr/hor/fdi:010040662
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers13-07/010040662.pdf
%V 77
%W Horizon (IRD)
%X To better understand the factors involved in maternal-fetal transmission of Trypanosoma cruzi, we compared DNA levels-obtained by use of quantitative real-time PCR and parasitic genotypes determined by PCR amplification followed by hybridization-in Bolivian mothers and their congenitally infected newborns. Mothers and their neonates displayed markedly different parasitic DNA levels, as most maternal estimated parasitemias (> 90%) were < 10 parasites/mL, whereas those of 76% of their newborns were > 1,000 parasites/mL. Comparison of T cruzi TeII sublineages infecting mothers and newborns showed identity, without evidence of mixed infection in mothers or neonates. Analysis of minor variants of TcIId-genotyped parasites using sequence class probes hybridizing with hypervariable domains of kDNA minicircles showed discrepancies in half of mother/newborn pairs.
%$ 052