@article{fdi:010040662,
  title = {{C}omparison of {T}rypanosoma cruzi lineages and levels of parasitic {DNA} in infected mothers and their newborns},
  author = {{V}irreira, {M}. and {T}ruyens, {C}. and {A}lonso {V}ega, {C}. and {B}rutus, {L}aurent and {J}ijena, {J}. and {T}orrico, {F}. and {C}arlier, {Y}ves and {S}voboda, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o better understand the factors involved in maternal-fetal transmission of {T}rypanosoma cruzi, we compared {DNA} levels-obtained by use of quantitative real-time {PCR} and parasitic genotypes determined by {PCR} amplification followed by hybridization-in {B}olivian mothers and their congenitally infected newborns. {M}others and their neonates displayed markedly different parasitic {DNA} levels, as most maternal estimated parasitemias (> 90%) were < 10 parasites/m{L}, whereas those of 76% of their newborns were > 1,000 parasites/m{L}. {C}omparison of {T} cruzi {T}e{II} sublineages infecting mothers and newborns showed identity, without evidence of mixed infection in mothers or neonates. {A}nalysis of minor variants of {T}c{II}d-genotyped parasites using sequence class probes hybridizing with hypervariable domains of k{DNA} minicircles showed discrepancies in half of mother/newborn pairs.},
  keywords = {},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {77},
  numero = {1},
  pages = {102--106},
  ISSN = {0002-9637},
  year = {2007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040662},
}