@article{fdi:010040644,
  title = {{T}he cell-cycle promoter cdc2a{A}t from {A}rabidopsis thaliana is induced in the lateral roots of the actinorhizal tree {A}llocasuarina verticillata during the early stages of the symbiotic interaction with {F}rankia},
  author = {{S}y, {M}ame {O}ur{\`e}ye and {H}ocher, {V}al{\'e}rie and {G}herbi, {H}assen and {L}aplaze, {L}aurent and {A}uguy, {F}lorence and {B}ogusz, {D}idier and {F}ranche, {C}laudine},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he symbiosis between the actinorhizal tree {A}llocasuarina verticillata and the actinomycete {F}rankia leads to the formation of root nodules inside which bacteria fix atmospheric nitrogen. {A}ctinorhizal nodule organogenesis starts with the induction of cell divisions in the root cortex and in the pericycle cells opposite protoxylem poles near {F}rankia-infected root hairs. {T}o study the ability of {F}rankia to induce progression through the cell cycle, we monitored the expression of the beta-glucuronidase (gus) gene driven by the promoter from cdc2a{A}t, an {A}rabidopsis cyclin-dependent kinase gene that displays competence for cell division, during plant growth and nodule ontogenesis. {I}n non-symbiotic tissues, the gus gene was mainly expressed in primary and secondary meristems of roots and shoots. {A}uxins and cytokinins were found to induce reporter gene activity in the root system of whole plants, showing that the promoter cdc2a{A}t displayed the same regulation by hormones in {A}llocasuarina as that reported in {A}rabidopsis. {I}n transgenic nodules, gus expression was found to be restricted to the phellogen. {D}uring the early stages of the interaction between {F}rankia and the plant root system, cdc2a{A}t was strongly induced in the lateral roots surrounded by hyphae of the actinomycete. {H}istochemical analysis of beta-glucuronidase activity revealed that cells from the pericycle opposite protoxylem poles were very deeply stained. {T}hese data indicate that upon {F}rankia infection, cells from the lateral roots, and notably pericycle cells that can give rise to a nodule or a root primordium, prepare to re-enter the cell cycle.},
  keywords = {},
  booktitle = {},
  journal = {{P}hysiologia {P}lantarum},
  volume = {130},
  numero = {3},
  pages = {409--417},
  ISSN = {0031-9317},
  year = {2007},
  DOI = {10.1111/j.1399-3054.2007.00884.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040644},
}