@article{fdi:010040642,
  title = {{M}olecular differentiation of three closely related members of the mosquito species complex, {A}nopheles moucheti, by mitochondrial and ribosomal {DNA} polymorphism},
  author = {{K}engne, {P}ierre and {A}ntonio {N}kondjio, {C}. and {A}wono {A}mbene, {H}. {P}. and {S}imard, {F}r{\'e}d{\'e}ric and {A}wolola, {T}. {S}. and {F}ontenille, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{D}istinction between members of the equatorial {A}frica malaria vector {A}nopheles moucheti ({E}vans) s.l. ({D}iptera: {C}ulicidae) has been based mainly on doubtful morphological features. {T}o determine the level of genetic differentiation between the three morphological forms of this complex, we investigated molecular polymorphism in the gene encoding for mitochondrial cytochrome oxidase b ({C}yt{B}) and in the ribosomal internal transcribed spacers ({ITS}1 and {ITS}2). {T}he three genomic regions revealed sequence differences between the three morphological forms similar in degree to the differences shown previously for members of other anopheline species groups or complexes (genetic distance d = 0.047-0.05 for {C}yt{B}, 0.084-0.166 for {ITS}1 and 0.03-0.05 for {ITS}2). {U}sing sequence variation in the {ITS}1 region, we set up a diagnostic polymerase chain reaction ({PCR}) for rapid and reliable identification of each subspecies within the {A}n. moucheti complex. {S}pecimens of {A}n. moucheti s.l. collected in {C}ameroon, the {D}emocratic {R}epublic of {C}ongo ({DRC}), {U}ganda and {N}igeria were successfully identified, demonstrating the general applicability of this technique.},
  keywords = {{A}nopheles moucheti ; allele specific {PCR} ; equatorial {A}frica ; malaria vector ; mitochondrial {DNA} ; ribosomal {DNA}},
  booktitle = {},
  journal = {{M}edical and {V}eterinary {E}ntomology},
  volume = {21},
  numero = {2},
  pages = {177--182},
  ISSN = {0269-283{X}},
  year = {2007},
  DOI = {10.1111/j.1365-2915.2007.00681.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040642},
}