@article{fdi:010040594,
  title = {{M}olecular characterization of an ethylene receptor gene ({C}c{ETR}1) in coffee trees, its relationship with fruit development and caffeine content},
  author = {{B}ustamante {P}orras, {J}. and {C}ampa, {C}laudine and {P}oncet, {V}al{\'e}rie and {N}oirot, {M}ichel and {L}eroy, {T}. and {H}amon, {S}erge and de {K}ochko, {A}lexandre},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o understand the importance of ethylene receptor genes in the quality of coffee berries three full-length c{DNA}s corresponding to a putative ethylene receptor gene ({ETR}1) were isolated from {C}offea canephora c{DNA} libraries. {T}hey differed by their 3'{UTR} and contained a main {ORF} and a 5'{UTR} short {ORF} putatively encoding a small polypeptide. {T}he {C}c{ETR}1 gene, present as a single copy in the {C}. canephora genome, contained five introns in the coding region and one in its 5'{UTR}. {A}lternative splicing can occur in {C}. canephora and {C}. pseudozanguebariae, leading to a truncated polypeptide. {C}. pseudozanguebariae {ETR}1 transcripts showed various forms of splicing alterations. {T}his gene was equally expressed at all stages of fruit development. {A} segregation study on an inter-specific progeny showed that {ETR}1 is related to the fructification time, the caffeine content of the green beans, and seed weight. {A}rabidopsis transformed etiolated seedlings, which over-expressed {C}c{ETR}1, displayed highly reduced gravitropism, but the triple response was observed in an ethylene enriched environment. {T}hese plants behaved like a low-concentration ethylene-insensitive mutant thus confirming the receptor function of the encoded protein. {T}his gene showed no induction during the climacteric crisis but some linkage with traits related to quality.},
  keywords = {coffee ; ethylene receptors ; caffeine content ; fruit ripening ; intron splicing ; overexpression},
  booktitle = {},
  journal = {{M}olecular {G}enetics and {G}enomics},
  volume = {277},
  numero = {6},
  pages = {701--712},
  ISSN = {1617-4615},
  year = {2007},
  DOI = {10.1007/s00438-007-0219-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040594},
}