@article{fdi:010037966,
  title = {{F}unctional characterization of two p-coumaroyl ester 3 '-hydroxylase genes from coffee tree : evidence of a candidate for chlorogenic acid biosynthesis},
  author = {{M}ahesh, {V}enkataramaiah and {M}illion {R}ousseau, {R}. and {U}llmann, {P}. and {C}habrillange, {N}athalie and {B}ustamante, {J}os{\'e} and {M}ondolot, {L}. and {M}orant, {M}. and {N}oirot, {M}ichel and {H}amon, {S}erge and {K}ochko, {A}lexandre de and {W}erck {R}eichhart, {D}. and {C}ampa, {C}laudine},
  editor = {},
  language = {{ENG}},
  abstract = {{C}hlorogenic acid (5-{CQA}) is one of the major soluble phenolic compounds that is accumulated in coffee green beans. {W}ith other hydroxycinnamoyl quinic acids ({HQA}s), this compound is accumulated in particular in green beans of the cultivated species {C}offea canephora. {R}ecent work has indicated that the biosynthesis of 5-{CQA} can be catalyzed by a cytochrome {P}450 enzyme, {CYP}98{A}3 from {A}rabidopsis. {T}wo full-length c{DNA} clones ({CYP}98{A}35 and {CYP}98{A}36) that encode putative p-coumaroylester 3'-hydroxylases ({C}3'{H}) were isolated from {C}. canephora c{DNA} libraries. {R}ecombinant protein expression in yeast showed that both metabolized p-coumaroyl shikimate at similar rates, but that only one hydroxylates the chlorogenic acid precursor p-coumaroyl quinate. {CYP}98{A}35 appears to be the first {C}3'{H} capable of metabolising p-coumaroyl quinate and p-coumaroyl shikimate with the same efficiency. {W}e studied the expression patterns of both genes on 4-month old {C}. canephora plants and found higher transcript levels in young and in highly vascularized organs for both genes. {G}ene expression and {HQA} content seemed to be correlated in these organs. {H}istolocalization and immunolocalization studies revealed similar tissue localization for caffeoyl quinic acids and p-coumaroylester 3'-hydroxylases. {T}he results indicated that {HQA} biosynthesis and accumulation occurred mainly in the shoot tip and in the phloem of the vascular bundles. {T}he lack of correlation between gene expression and {HQA} content observed in some organs is discussed in terms of transport and accumulation mechanisms.},
  keywords = {caffeoyl quinic acids ; chlorogenic acid ; {C}offea canephora ; cytochrome {P}450 hydroxylase},
  booktitle = {},
  journal = {{P}lant {M}olecular {B}iology},
  volume = {64},
  numero = {1-2},
  pages = {145--159},
  ISSN = {0167-4412},
  year = {2007},
  DOI = {10.1007/s11103-007-9141-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037966},
}