@article{fdi:010037846,
  title = {{D}evelopment of a one-step immunochromatographic strip test for the rapid detection of nevirapine ({NVP}), a commonly used antiretroviral drug for the treatment of {HIV}/{AIDS}},
  author = {{P}attarawarapan, {M}. and {N}angola, {S}. and {C}ressey, {T}imothy {R}. and {T}ayapiwatana, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}urrently, high-performance liquid chromatographic ({HPLC}) methods are mainly used to measure anfiretroviral plasma concentrations in {HIV}-infected patients. {A}lthough the utility of routine therapeutic drug monitoring ({TDM}) as an additional toot to optimize long-term antiretroviral therapy is unclear, if {TDM} is to be widely used, the availability of simple, cheap and reliable methods for the measurement of antiretroviral drug levels are needed, particularly in resource-limited settings. {I}n this study, an immunochromatograhic ({IC}) strip test to detect the presence of nevirapine ({NVP}) in body fluids has been developed. {A}ntiserum to {NVP} was first raised in rabbits by immunization against {NVP} chemically conjugated with bovine serum albumin, and subsequently validated by {W}estern immunoblotting and competitive indirect {ELISA}. {T}he partially purified anti-{NVP} antibodies were conjugated with colloidal gold particles. {T}he conjugation of the colloidal gold and polyclonal antibodies was monitored by {UV}-vis spectroscopy, while transmission electron microscopy images were used to characterize the particle size and shape of the conjugates, {T}he resulting colloidal gold conjugates were used for the production of an {IC} strip test to detect nevirapine in human plasma. {P}reliminary assessment suggests no-cross reactivity of the {NVP} polyclonal antibodies but assessment of plasma samples from {HIV}-infected patients receiving {HAART} needs to be conducted. {T}his assay could potentially be used for drug monitoring as part of the clinical care of {HIV} infected patients.},
  keywords = {{NVP} ; immunochromatographic strip test ; colloidal gold ; immunoassay},
  booktitle = {},
  journal = {{T}alanta},
  volume = {71},
  numero = {1},
  pages = {462--470},
  ISSN = {0039-9140},
  year = {2007},
  DOI = {10.1016/j.talanta.2006.05.059},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037846},
}