@article{fdi:010037842,
  title = {{A} biological test to quantify pyrethroid in impregnated nets},
  author = {{M}artin, {T}hibaud and {C}handre, {F}abrice and {C}habi, {J}. and {G}uillet, {P}ierre {F}. and {A}kogbeto, {M}. and {H}ougard, {J}ean-{M}arc},
  editor = {},
  language = {{ENG}},
  abstract = {{F}irst instar larvae of {A}edes aegypti ({L}.) ({D}iptera: {C}ulicidae) are very susceptible to deltamethrin, which kills all larvae at a very low dose (0.1 mu g/l). {T}hus the sensitivity of this insect to detect that pyrethroid is in parts per billion, as obtained with most common chromatographic methods of analysis. {H}ere we describe a biological test ({BT}) to quantify deltamethrin in long-lasting insecticidal nets ({LLIN}s) by exposing first instar larvae of {A}e. aegypti to serial extracted insecticide solutions from net samples. {T}he deltamethrin concentration in the net was calculated at the doses killing 50% of larva, from the {LC}50 of deltamethrin (6.5 x 10(-5) mg/l) and the dilution factor ({DF}50) of the extracted net solution. {T}he pyrethroid quantification in {LLIN}s after 0-25 washes with this {BT} was correlated with those obtained by direct chromatographic analysis (r(2) = 0.84). {T}his {BT} did not require sophisticated equipment and could be extended to other molecules and materials. {I}t appeared accurate, robust, cheap and well adapted to the national malaria programmes as the eggs of {A}e. aegypti might be used for some months. {T}his method was adapted to provide an easy to use kit test for the quality control of {LLIN}s in the field.},
  keywords = {insecticide treated net ; long lasting insecticidal nets ; deltamethrin ; biological analysis ; {A}edes aegypti},
  booktitle = {},
  journal = {{T}ropical {M}edicine and {I}nternational {H}ealth},
  volume = {12},
  numero = {2},
  pages = {245--250},
  ISSN = {1360-2276},
  year = {2007},
  DOI = {10.1111/j.1365-3156.2006.01777.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037842},
}