Kim H. H. auteur aut IRD Lee J. K. auteur aut IRD Yoon J. W. auteur aut IRD Ji J. J. auteur aut IRD Nam S. S. auteur aut IRD Hwang H. S. auteur aut IRD Cho E. G. auteur aut IRD Engelmann Florent auteur aut IRD text journalArticle eng text/pdf born digital access The droplet-vitrification protocol, a combination of droplet-freezing and solution-based vitrification was applied for cryopreserving garlic bulbil primordia. The highest survival and regeneration percentages of cryopreserved primordia (90.1-95.0% and 82.7-85.0%, respectively) were achieved after preculture for 2-4 days at 10 degrees C on solid medium with 0.1-0.3 M sucrose, loading for 50 minutes in liquid medium with 2 M glycerol + 0.5 M sucrose, dehydration with PVS3 vitrification solution for 90-150 min, cooling primordia in 5 mu l droplets of-PVS3 vitrification solution placed on aluminum foil strips by dipping these strips in liquid nitrogen, warming them by plunging the foil strips into pre-heated (40 degrees C) 0.8 M sucrose solution for 30 s and further incubation in the same solution for 30 minutes. The optimized droplet-vitrification protocol was successfully applied to bulbil primordia of five garlic varieties originating from various countries and to immature bulbils of two vegetatively propagated Allium species, with regeneration percentages ranging between 77.4-95.4%. specialized Allium sativum L droplet vitrification bulbil primordium cooling warming 076 27 3 143-153 2006 0143-2044 https://www.documentation.ird.fr/hor/fdi:010037800 0143-2044 [F B010037800] https://www.documentation.ird.fr/hor/fdi:010037800 https://www.documentation.ird.fr/intranet/publi/2007/suppl/010037800.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2007-09-06 2017-08-23 fdi:010037800 fre