@article{fdi:010037800,
  title = {{C}ryopreservation of garlic bulbil primordia by the droplet-vitrification procedure},
  author = {{K}im, {H}. {H}. and {L}ee, {J}. {K}. and {Y}oon, {J}. {W}. and {J}i, {J}. {J}. and {N}am, {S}. {S}. and {H}wang, {H}. {S}. and {C}ho, {E}. {G}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he droplet-vitrification protocol, a combination of droplet-freezing and solution-based vitrification was applied for cryopreserving garlic bulbil primordia. {T}he highest survival and regeneration percentages of cryopreserved primordia (90.1-95.0% and 82.7-85.0%, respectively) were achieved after preculture for 2-4 days at 10 degrees {C} on solid medium with 0.1-0.3 {M} sucrose, loading for 50 minutes in liquid medium with 2 {M} glycerol + 0.5 {M} sucrose, dehydration with {PVS}3 vitrification solution for 90-150 min, cooling primordia in 5 mu l droplets of-{PVS}3 vitrification solution placed on aluminum foil strips by dipping these strips in liquid nitrogen, warming them by plunging the foil strips into pre-heated (40 degrees {C}) 0.8 {M} sucrose solution for 30 s and further incubation in the same solution for 30 minutes. {T}he optimized droplet-vitrification protocol was successfully applied to bulbil primordia of five garlic varieties originating from various countries and to immature bulbils of two vegetatively propagated {A}llium species, with regeneration percentages ranging between 77.4-95.4%.},
  keywords = {{A}llium sativum {L} ; droplet vitrification ; bulbil primordium ; cooling ; warming},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {27},
  numero = {3},
  pages = {143--153},
  ISSN = {0143-2044},
  year = {2006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037800},
}