@article{fdi:010037793,
  title = {{C}ryopreservation of cultivated and wild potato varieties by droplet vitrification : {E}ffect of subculture of mother-plants and of preculture of shoot tips},
  author = {{Y}oon, {J}. {W}. and {K}im, {H}. {H}. and {K}o, {H}. {C}. and {H}wang, {H}. {S}. and {H}ong, {E}. {S}. and {C}ho, {E}. {G}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this paper, we studied the effect of subculture of mother-plants and of preculture of shoot tips of two potato varieties ({D}ejima, cultivated and {STN}13, wild) cryopreserved using the droplet-vitrification technique. {T}he subculture conditions, i.e. light intensity, aeration and planting density significantly affected survival of both non-cryopreserved and cryopreserved shoot-tips in both varieties. {T}he subculture duration and the position of the shoot tips on the axis of the in vitro plantlets had a significant ({P} < 0.0001) effect on survival of cryopreserved shoot tips. {T}he optimal subculture duration was 7 and 5 weeks and the optimal size of shoot tips was 1.5-2.0 and 1.0-1.5 mm for var. {D}ejima and {STN}13, respectively. {S}urvival of cryopreserved shoot tips was influenced by the sucrose concentration in the preculture medium and the preculture duration. {T}he highest survival of cryopreserved shoot tips was observed after preculture with 0.3 {M} sucrose for 8 h followed by 0.7 {M} sucrose for 18 h. {T}hese results indicate that the parameters of the subculture of mother-plants and of preculture of shoot tips should be carefully optimized, especially in the case of wild species.},
  keywords = {droplet vitrification ; {S}olanum tuberosum {L} ; shoot tips ; subculture ; preculture},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {27},
  numero = {4},
  pages = {211--222},
  ISSN = {0143-2044},
  year = {2006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037793},
}