LoPPS : A long PCR product sequencing method for rapid characterisation of long amplicons /Emonet, Sébastien Grard, G. Brisbarre, N. Moureau, G. Temmam, S. Charrel, R. /de Lamballerie, Xavier sequencing PCR RNA viruses LoPPS Here, we propose an optimised protocol (LoPPS, long PCR product sequencing) which allows the fast, cost-attractive, and high-throughput sequencing of long PCR products. LoPPS constitutes an alternative to the primer-walking technology which is expensive and time consuming but remains the current standard procedure. It is based on the ultrasonic shearing, polishing, and cloning of PCR or RT-PCR products and is compatible with 96- or 384-well microplate systems in which bacterial growth, preparation of plasmid DNA, and sequencing can be automated. We present results obtained from 24 different RT-PCR products (2.5-4.8 kbp long) obtained from various RNA viruses and fully sequenced using LoPPS. The method proved to be robust and fast. It was successfully used on a low amount of DNA and allowed each target nucleotide position to be controlled twice or more, with a final cost which is one-third of that of primer-walking. 2006 text https://www.documentation.ird.fr/hor/fdi:010037790 fdi:010037790 Emonet Sébastien, Grard G., Brisbarre N., Moureau G., Temmam S., Charrel R., de Lamballerie Xavier. LoPPS : A long PCR product sequencing method for rapid characterisation of long amplicons. 2006, 344 (4), 1080-1085 EN