@article{fdi:010037790,
  title = {{L}o{PPS} : {A} long {PCR} product sequencing method for rapid characterisation of long amplicons},
  author = {{E}monet, {S}{\'e}bastien and {G}rard, {G}. and {B}risbarre, {N}. and {M}oureau, {G}. and {T}emmam, {S}. and {C}harrel, {R}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{H}ere, we propose an optimised protocol ({L}o{PPS}, long {PCR} product sequencing) which allows the fast, cost-attractive, and high-throughput sequencing of long {PCR} products. {L}o{PPS} constitutes an alternative to the primer-walking technology which is expensive and time consuming but remains the current standard procedure. {I}t is based on the ultrasonic shearing, polishing, and cloning of {PCR} or {RT}-{PCR} products and is compatible with 96- or 384-well microplate systems in which bacterial growth, preparation of plasmid {DNA}, and sequencing can be automated. {W}e present results obtained from 24 different {RT}-{PCR} products (2.5-4.8 kbp long) obtained from various {RNA} viruses and fully sequenced using {L}o{PPS}. {T}he method proved to be robust and fast. {I}t was successfully used on a low amount of {DNA} and allowed each target nucleotide position to be controlled twice or more, with a final cost which is one-third of that of primer-walking.},
  keywords = {sequencing ; {PCR} ; {RNA} viruses ; {L}o{PPS}},
  booktitle = {},
  journal = {{B}iochemical and {B}iophysical {R}esearch {C}ommunications},
  volume = {344},
  numero = {4},
  pages = {1080--1085},
  ISSN = {0006-291{X}},
  year = {2006},
  DOI = {10.1016/j.bbrc.2006.04.015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037790},
}