@article{fdi:010037782,
  title = {{C}lonal propagation and the fast generation of karyotype diversity: an in vitro {L}eishmania model},
  author = {{D}ujardin, {J}. {C}. and {D}e {D}oncker, {S}. and {J}acquet, {D}. and {B}añuls, {A}nne-{L}aure and {B}alavoine, {M}. and {V}an {B}ockstaele, {D}. and {T}ibayrenc, {M}ichel and {A}revalo, {J}. and {L}e {R}ay, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n the present work we studied the karyotype stability during long-term in vitro maintenance in 3 cloned strains of {L}eishmania ({V}iannia) peruviana, {L}eishmania ({V}iannia) braziliensis and a hybrid between both species. {O}nly the {L}. ({V}.) peruviana strain showed an unstable karyotype, even after subcloning. {F}our chromosomes were studied in detail, each of them characterized by homologous chromosomes of different size (heteromorphy). {V}ariations in chromosome patterns during in vitro maintenance were rapid and discrete, involving loss of heteromorphy or appearance of additional chromosome size variants. {T}he resulting pattern was not the same according to experimental conditions (subinoculation rate or incubation temperature), and interestingly, this was associated with differences in growth behaviour of the respective parasites. {N}o change in total ploidy of the cells was observed by flow cytometry. {W}e discuss several mechanisms that might account for this variation of chromosome patterns, but we favour the occurrence of aneuploidy, caused by aberrant chromosome segregation during mitosis. {O}ur results provide insight into the generation of karyotype diversity in natural conditions and highlight the relativity of the clone concept in parasitology.},
  keywords = {{L}eishmania {V}iannia peruviana ; karvotype ; hybrid ; selection ; ploidy ; pseudo sexuality},
  booktitle = {},
  journal = {{P}arasitology},
  volume = {134},
  numero = {1},
  pages = {33--39},
  ISSN = {0031-1820},
  year = {2007},
  DOI = {10.1017/{S}0031182006001156},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037782},
}