%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Ortega Morales, B. O.
%A Santiago Garcia, J. L.
%A Chan Bacab, M. J.
%A Moppert, X.
%A Miranda Tello, Elizabeth
%A Fardeau, Marie-Laure
%A Carrero, J. C.
%A Bartolo Perez, P.
%A Valadez Gonzalez, A.
%A Guezennec, J.
%T Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria
%D 2007
%L fdi:010037744
%G ENG
%J Journal of Applied Microbiology
%@ 1364-5072
%K anionic ; Bacillus sp ; exopolymers ; Microbacterium sp ; surfactants ; tropical intertidal biofilm bacteria
%M CC:0002428329-0029
%N 1
%P 254-264
%R 10.1111/j.1365-2672.2006.03085.x
%U https://www.documentation.ird.fr/hor/fdi:010037744
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-12/010037744.pdf
%V 102
%W Horizon (IRD)
%X Aims: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. EPS MC3B-10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. The chemical composition of polymer MC6B-22 suggests its potential biomedical application in tissue regeneration. Significance and Impact of the Study: This is the first report of a Microbacterium species producing EPS with surfactant properties, which expands our knowledge of the micro-organisms capable of producing these biomolecules. Furthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting EPS-producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication.
%$ 084