@article{fdi:010037744, title = {{C}haracterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria}, author = {{O}rtega {M}orales, {B}. {O}. and {S}antiago {G}arcia, {J}. {L}. and {C}han {B}acab, {M}. {J}. and {M}oppert, {X}. and {M}iranda {T}ello, {E}lizabeth and {F}ardeau, {M}arie-{L}aure and {C}arrero, {J}. {C}. and {B}artolo {P}erez, {P}. and {V}aladez {G}onzalez, {A}. and {G}uezennec, {J}.}, editor = {}, language = {{ENG}}, abstract = {{A}ims: {T}his study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers ({EPS}). {M}ethods and {R}esults: {A} screening procedure was implemented to detect {EPS}-producing biofilm bacteria. {I}solates {MC}3{B}-10 and {MC}6{B}-22, identified respectively as a {M}icrobacterium species and {B}acillus species by 16{S} r{DNA} and cellular fatty acids analyses, produced different {EPS}, as evidenced by colorimetric and gas chromatographic analyses. {T}he polymer produced by isolate {MC}3{B}-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. {C}onclusions: {P}olymer {MC}3{B}-10 appears to be a glycoprotein, while {EPS} {MC}6{B}-22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. {EPS} {MC}3{B}-10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. {T}he chemical composition of polymer {MC}6{B}-22 suggests its potential biomedical application in tissue regeneration. {S}ignificance and {I}mpact of the {S}tudy: {T}his is the first report of a {M}icrobacterium species producing {EPS} with surfactant properties, which expands our knowledge of the micro-organisms capable of producing these biomolecules. {F}urthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting {EPS}-producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication.}, keywords = {anionic ; {B}acillus sp ; exopolymers ; {M}icrobacterium sp ; surfactants ; tropical intertidal biofilm bacteria}, booktitle = {}, journal = {{J}ournal of {A}pplied {M}icrobiology}, volume = {102}, numero = {1}, pages = {254--264}, ISSN = {1364-5072}, year = {2007}, DOI = {10.1111/j.1365-2672.2006.03085.x}, URL = {https://www.documentation.ird.fr/hor/fdi:010037744}, }