@article{fdi:010037709,
  title = {{I}ncreased melanizing activity in {A}nopheles gambiale does not affect development of {P}lasmodium falciparum},
  author = {{M}ichel, {K}. and {S}uwanchaichinda, {C}. and {M}orlais, {I}sabelle and {L}ambrechts, {L}. and {C}ohuet, {A}nna and {A}wono-{A}mbene, {P}. and {S}imard, {F}r{\'e}d{\'e}ric and {F}ontenille, {D}idier and {K}anost, {M}.{R}. and {K}afatos, {F}.{C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}erpins are central to the modulation of various innate immune responses in insects and are suspected to influence the outcome of malaria parasite infection in mosquito vectors. {T}hree {A}nopheles gambiae serpins ({SRPN}1, -2, and -3) were tested for their ability to inhibit the prophenoloxidase cascade, a key regulatory process in the melanization response. {R}ecombinant {SPRN}1 and -2 can bind and inhibit a heterologous phenoloxiclase-activating protease and inhibit phenoloxidase activation in vitro. {U}sing a reverse genetics approach, we studied the effect of {SRPN}2 on melanization in {A}n. gambiae adult females in vivo. {D}epletion of {SRPN}2 from the mosquito hemolymph increases melanin deposition on foreign surfaces such as negatively charged {S}ephadex beads. {A}s reported, the knockdown of {SRPN}2 adversely affects the ability of the rodent malaria parasite {P}lasmodium berghei to invade the midgut epithelium and develop into oocysts. {I}mportantly, we tested whether the absence of {SRPN}2 from the hemolymph influences {P}lasmodium falciparum development. {RNA}i silencing of {SRPN}2 in an {A}n. gambiae strain originally established from local populations in {Y}aounde, {C}ameroon, did not influence the development of autochthonous field isolates of {P}. falciparum. {T}his study suggests immune evasion strategies of the human malaria parasite and emphasizes the need to study mosquito innate immune responses toward the pathogens they transmit in natural vector-parasite combinations.},
  keywords = {innate immunity ; malaria ; mosquito ; serpin},
  booktitle = {},
  journal = {{P}roceedings of the {N}ational {A}cademy of {S}ciences of the {U}nited {S}tates of {A}merica},
  volume = {103},
  numero = {45},
  pages = {16858--16863},
  ISSN = {0027-8424},
  year = {2006},
  DOI = {10.1073/pnas.0608033103},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037709},
}