@article{fdi:010037608,
  title = {{D}evelopment of a lab-made microarray for analyzing the genetic diversity of nitrogen fixing symbionts {S}inorhizobium meliloti and {S}inorhizobium medicae},
  author = {{B}ailly, {X}avier and {B}{\'e}na, {G}illes and {L}enief, {V}anina and {L}ajudie, {P}hilippe de and {A}varre, {J}ean-{C}hristophe},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ome bacterial species, like nitrogen-fixing {S}inorhizobium that interact with {M}edicago plants, are prone to frequent horizontal gene transfers. {I}nvestigation of their genetic structure requires to study polymorphism patterns at many loci. {A}lthough {DNA} microarrays represent a method of choice for high throughput analysis of polymorphisms, this technology yet remains an expensive and heavy approach, thus depriving most of research groups from this powerful tool. {I}n an attempt to overcome this limitation, we have developed a simple genotyping procedure by {DNA} microarrays, and have evaluated its ability to characterize a {S}inorhizobium population. {T}hirty 18- to 24-mer oligonucleotide probes were designed to target the most frequent mutations in three polymorphic loci of {S}inorhizobium meliloti and {S}. medicae. {P}robe hybridization efficiency was compared on two spotting surfaces: nylon membranes and epoxy-coated glass slides. {E}poxy-coated glass slides revealed more sensitive than nylon membranes and allowed discrimination of single mismatches. {U}sing this procedure, an uncharacterized population consisting of 33 {S}. meliloti/{S}. medicae isolates was successfully genotyped. (c) 2006 {E}lsevier {B}.{V}. {A}ll rights reserved.},
  keywords = {microarray ; array surface ; genotyping ; {S}inorhizobium},
  booktitle = {},
  journal = {{J}ournal of {M}icrobiological {M}ethods},
  volume = {67},
  numero = {1},
  pages = {114--124},
  ISSN = {0167-7012},
  year = {2006},
  DOI = {10.1016/j.mimet.2006.03.006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010037608},
}