Leroy T. auteur aut IRD Marraccini P. auteur aut IRD Dufour M. auteur aut IRD Montagnon C. auteur aut IRD Lashermes Philippe auteur aut IRD Sabau X. auteur aut IRD Ferreira L.P. auteur aut IRD Jourdan I. auteur aut IRD Pot D. auteur aut IRD Andrade A.C. auteur aut IRD Glaszmann J.C. auteur aut IRD Vieira L.G.E. auteur aut IRD Piffanelli P. auteur aut IRD text journalArticle eng The first bacterial artificial chromosome (BAC) library of Robusta coffee (Coffea canephora) was constructed, with the aim of developing molecular resources to study the genome structure and evolution of this perennial crop. Clone 126, which is highly productive and confers good technological and organoleptic qualities of beverage, was chosen for development of this library. The BAC library contains 55,296 clones, with an average insert size of 135 Kb per plasmid, therefore representing theoretically nine haploid genome equivalents of C. canephora. Its validation was achieved with a set of 13 genetically anchored single-copy and 4 duplicated RFLP probes and yielded on average 9 BAC clones per probe. Screening of this BAC library was also carried out with partial cDNA probes coding for enzymes of sugar metabolism like invertases and sucrose synthase, with the aim of characterizing the organization and promoter structure of this important class of genes. It was shown that genes for both cell wall and vacuolar forms of invertases were probably unique in the Robusta genome whereas sucrose synthase was encoded by at least two genes. One of them (CcSUS1) was cloned and sequenced, showing that our BAC library is a valuable tool to rapidly identify genes of agronomic interest or linked to cup quality in C. canephora. specialized 076 111 6 1032-1041 2005 0040-5752 https://www.documentation.ird.fr/hor/fdi:010036710 10.1007/s00122-005-0018-z 0040-5752 [F B010036710] https://www.documentation.ird.fr/hor/fdi:010036710 IRD - Base Horizon / Pleins textes 2006-03-29 2017-08-23 fdi:010036710 fre