@article{fdi:010036709,
  title = {{C}omparison of four constitutive promoters for the expression of transgenes in the tropical nitrogen-fixing tree {A}llocasuarina verticillata},
  author = {{O}bertello, {M}ariana and {S}anti, {C}arole and {S}y, {M}ame {O}ureye and {L}aplaze, {L}aurent and {A}uguy, {F}lorence and {B}ogusz, {D}idier and {F}ranche, {C}laudine},
  editor = {},
  language = {{ENG}},
  abstract = {{A}llocasuarina verticillata is an actinorhizal tree that lives in symbiotic association with a nitrogen fixing actinomycete called {F}rankia. {I}n the search for promoters that drive strong constitutive expression in this tropical tree, we studied the organ specificity of four different constitutive promoters ({C}a{MV} 35{S}, e35{S}, e35{S}-4ocs and {UBQ}1 from {A}rabidopsis thaliana) in stably transformed {A}. verticillata plants. {T}he beta-glucuronidase (gus) gene was used as a reporter and expression studies were carried out by histochemical analyses on shoots, roots and actinorhizal nodules. {W}hile the 35{S} promoter was poorly expressed in the shoot apex and lateral roots, both the e35{S} and e35{S}-4ocs were found to drive high constitutive expression in the transgenic non-nodulated plants. {I}n contrast, the {UBQ}1 promoter was very poorly expressed and appeared unsuitable for {A}. verticillata. {W}e also showed that none of the promoters studied were active in the nodule infected cells, whatever the developmental stage studied.},
  keywords = {{A}llocasuarina verticillata ; actinorhizal plant ; beta glucuronidase ; promoter ; nitrogen fixing nodules},
  booktitle = {},
  journal = {{P}lant {C}ell {R}eports},
  volume = {24},
  numero = {9},
  pages = {540--548},
  ISSN = {0721-7714},
  year = {2005},
  DOI = {10.1007/s00299-005-0963-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010036709},
}