@article{fdi:010035840,
  title = {{E}xpressed sequence-tag analysis in {C}asuarina glauca actinorhizal nodule and root},
  author = {{H}ocher, {V}al{\'e}rie and {A}uguy, {F}lorence and {A}rgout, {X}. and {L}aplaze, {L}aurent and {F}ranche, {C}laudine and {B}ogusz, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he present study aimed to identify and assess the frequency and tissue specificity of plant genes in the actinorhizal {C}asuarina glauca-{F}rankia symbiosis through expressed sequence tag ({EST}) analysis. {U}sing a custom analysis pipeline for raw sequences of {C}. glauca uninfected roots and nodules, we obtained an {EST} databank web interface. {G}ene expression was studied in nodules vs roots using comparative quantitative real-time reverse transcription-polymerase chain reaction (q{RT}-{PCR}). {F}rom roots and nodules, 2028 {EST}s were created and clustered in 242 contigs and 1429 singletons, giving a total of 1616 unique genes. {H}alf the nodule transcripts showed no similarity to previously identified genes. {G}enes of primary metabolism, protein synthesis, cell division and defence were highly represented in the nodule library. {D}ifferential expression was observed between roots and nodules for several genes linked to primary metabolism and flavonoid biosynthesis. {T}his comparative {EST}-based study provides the first picture of the set of genes expressed during actinorhizal symbiosis. {W}e consider our database to be a flexible tool that can be used for the management of {EST} data from other actinorhizal symbioses.},
  keywords = {actinorhizal symbiosis ; {C}asuarina glauca ; {F}rankia ; expressed sequence tags {EST}s ; quantitative real time {RT} {PCR} q{RT} {PCR}},
  booktitle = {},
  journal = {{N}ew {P}hytologist},
  volume = {169},
  numero = {4},
  pages = {681--688},
  ISSN = {0028-646{X}},
  year = {2006},
  DOI = {10.1111/j.1469-8137.2006.01644.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010035840},
}