Mahesh Venkataramaiah auteur aut IRD Rakotomalala Jean-Jacques auteur aut IRD Le Gal Lénaïg auteur aut IRD Vigne Hélène auteur aut IRD De Kochko Alexandre auteur aut IRD Hamon Serge auteur aut IRD Noirot Michel auteur aut IRD Campa Claudine auteur aut IRD text journalArticle eng text/pdf born digital access Biosynthesis of caffeoylquinic acids occurs via the phenylpropanoid pathway in which the phenylalanine ammonia-lyase (PAL) acts as a key-control enzyme. A full-length cDNA (pF6), corresponding to a PAL gene (CcPAL1), was isolated by screening a Coffea canephora fruit cDNA library and its corresponding genomic sequence was characterized. Amplification of total DNA from seven Coffea species revealed differences in intronic length. This interspecific polymorphism was used to locate the gene on a genetic map established for a backcross progeny between Coffea pseudozanguebariae and C. dewevrei. The CcPAL1 gene was found on the same linkage group, but genetically independent, as a caffeoyl-coenzyme A-O-methyltransferase gene, another gene intervening in the phenylpropanoid pathway. In the same backcross, a lower caffeoylquinic acid content was observed in seeds harvested from plants harbouring the C. pseudozanguebariae CcPAL1 allele. Involvement of the CcPAL1 allelic form in the differential accumulation of caffeoylquinic acids in coffee green beans is then discussed. specialized chlorogenic acid Coffea genetic diversity gene structure phenylalanine ammonia lyase 076 25 9 986-992 2006 0721-7714 https://www.documentation.ird.fr/hor/fdi:010035762 10.1007/s00299-006-0152-3 0721-7714 [F B010035762] https://www.documentation.ird.fr/hor/fdi:010035762 https://www.documentation.ird.fr/intranet/publi/2006/09/010035762.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2006-10-06 2017-08-23 fdi:010035762 fre