@article{fdi:010035762, title = {{I}solation and genetic mapping of a {C}offea canephora phenylalanine ammonia-lyase gene ({C}c{PAL}1) and its involvement in the accumulation of caffeoyl quinic acids}, author = {{M}ahesh, {V}enkataramaiah and {R}akotomalala, {J}ean-{J}acques and {L}e {G}al, {L}{\'e}na{\¨ie}g and {V}igne, {H}{\'e}l{\`e}ne and {D}e {K}ochko, {A}lexandre and {H}amon, {S}erge and {N}oirot, {M}ichel and {C}ampa, {C}laudine}, editor = {}, language = {{ENG}}, abstract = {{B}iosynthesis of caffeoylquinic acids occurs via the phenylpropanoid pathway in which the phenylalanine ammonia-lyase ({PAL}) acts as a key-control enzyme. {A} full-length c{DNA} (p{F}6), corresponding to a {PAL} gene ({C}c{PAL}1), was isolated by screening a {C}offea canephora fruit c{DNA} library and its corresponding genomic sequence was characterized. {A}mplification of total {DNA} from seven {C}offea species revealed differences in intronic length. {T}his interspecific polymorphism was used to locate the gene on a genetic map established for a backcross progeny between {C}offea pseudozanguebariae and {C}. dewevrei. {T}he {C}c{PAL}1 gene was found on the same linkage group, but genetically independent, as a caffeoyl-coenzyme {A}-{O}-methyltransferase gene, another gene intervening in the phenylpropanoid pathway. {I}n the same backcross, a lower caffeoylquinic acid content was observed in seeds harvested from plants harbouring the {C}. pseudozanguebariae {C}c{PAL}1 allele. {I}nvolvement of the {C}c{PAL}1 allelic form in the differential accumulation of caffeoylquinic acids in coffee green beans is then discussed.}, keywords = {chlorogenic acid ; {C}offea ; genetic diversity ; gene structure ; phenylalanine ammonia lyase}, booktitle = {}, journal = {{P}lant {C}ell {R}eports}, volume = {25}, numero = {9}, pages = {986--992}, ISSN = {0721-7714}, year = {2006}, DOI = {10.1007/s00299-006-0152-3}, URL = {https://www.documentation.ird.fr/hor/fdi:010035762}, }