@article{fdi:010035732,
  title = {{Q}uantitation of {HIV}-1 {RNA} in breast milk by real time {PCR}},
  author = {{B}ecquart, {P}ierre and {F}oulongne, {V}incent and {W}illumsen, {J}. and {R}ouzioux, {C}. and {S}egondy, {M}ichel and {V}an de {P}erre, {P}hilippe},
  editor = {},
  language = {{ENG}},
  abstract = {{HIV}-1 {RNA} in breast milk is a strong predictor of {HIV}-1 transmission through breastfeeding. {I}n the present report, breast milk samples from {HIV}-1 uninfected donors were spiked with dilution of quantified culture supernatant from {HIV}-1({NDK}) infected {PBMC}. {T}wo {RNA} extraction techniques based on silica extraction, {N}uclisens ({R}) ({B}io{M}erieux) and {T}riazol ({Q}iagen), two techniques based on guanidine thiocynanate/chloroforme extraction, {TRI}zol ({L}ife {T}echnologic) and {A}mplicor {HIV}-1 {M}onitor ({TM}) ({R}oche {D}iagnostic {S}ystems), and one technique based on electrostatic adsorption on iron oxide micro beads ({P}romega) were compared. {HIV}-1 {RNA} was quantitated by real time {PCR} ({LTR} gene) and {A}mplicor {HIV}-1 {M}onitor ({TM}). {C}ombining magnetic micro beads extraction and real time {PCR} quantitation allowed to correctly quantify breast milk {HIV}-1 {RNA}, with a difference between the expected and measured {HIV}-1 {RNA} levels always lower than 0.3 log copies/mi. {T}he same combination was confirmed on 25 breast milk samples from {HIV}-1 infected women collected in {K}wazulu-{N}atal, {S}outh {A}frica, by comparing measurements with those obtained by the {A}mplicor {HIV}-1 {M}onitor ({TM}) (r(2) = 0.88). {N}ucleic acid extraction by magnetic micro beads followed by real time {PCR} is a reliable, sensitive, rapid and simple procedure to quantify {HIV}-1 {RNA} in breast milk and allows for {PCR} inhibitors found frequently in these samples. (c) 2005 {E}lsevier {B}.{V}. {A}ll rights reserved.},
  keywords = {real time {PCR} ; {HIV} 1 {RNA} quantitation ; breast milk},
  booktitle = {},
  journal = {{J}ournal of {V}irological {M}ethods},
  volume = {133},
  numero = {1},
  pages = {109--111},
  ISSN = {0166-0934},
  year = {2006},
  DOI = {10.1016/j.jviromet.2005.10.022},
  URL = {https://www.documentation.ird.fr/hor/fdi:010035732},
}