@article{fdi:010035729,
  title = {{E}fficient production of {A}grobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots},
  author = {{A}lpizar, {E}. and {D}echamp, {E}. and {E}speout, {S}. and {R}oyer, {M}. and {L}ecouls, {A}nne-{C}laire and {N}icole, {M}ichel and {B}ertrand, {B}. and {L}ashermes, {P}hilippe and {E}tienne, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he possibility of rapid validation and functional analysis of nematode resistance genes is a common objective for numerous species and particularly for woody species. {I}n this aim, we developed an {A}grobacterium rhizogenes-mediated transformation protocol for {C}offea arabica enabling efficient and rapid regeneration of transformed roots from the hypocotyls of germinated zygotic embryos, and the subsequent production of composite plants. {T}he {A}. rhizogenes strain {A}4{RS} proved to be the most virulent. {H}igh transformation efficiencies (70%) were obtained using a 2-week co-cultivation period at a temperature of 15-18 degrees {C}. {U}sing a p35{S}-gus{A}-int construct inserted in the p{BIN}19 binary plasmid, we could estimate that 35% of transformed roots were {GUS} positive (co-transformed). {U}sing the {GUS} assay as visual marker, 40% composite plants bearing a branched co-transformed rootstock could be obtained after only 12 weeks without selection with herbicides or antibiotics. {T}ransgenic coffee roots obtained with {A}. rhizogenes did not exhibit the 'hairy' disturbed phenotype and were morphologically similar to normal roots. {PCR} analyses demonstrated that all co-transformed roots were positive for the expected rol{B} and gus{A} genes. {T}ransformed and non-transformed root systems from both susceptible and resistant varieties were inoculated with {M}eloidogyne exigua nematode individuals. {I}noculation of composite plants from the {C}aturra susceptible variety resulted in the normal development of nematode larvae. {N}umbers of extracted nematodes demonstrated that transformed roots retain the resistance/sensibility phenotype of varieties from which they are derived. {T}hese results suggest that composite plants constitute a powerful tool for studying nematode resistance genes.},
  keywords = {{A}grobacterium rhizogenes ; coffee ; composite plant ; genetic transformation ; nematode},
  booktitle = {},
  journal = {{P}lant {C}ell {R}eports},
  volume = {25},
  numero = {9},
  pages = {959--967},
  ISSN = {0721-7714},
  year = {2006},
  DOI = {10.1007/s00299-006-0159-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010035729},
}