@article{fdi:010035716,
  title = {{E}ffect of primary culture medium composition on high frequency somatic embryogenesis in different {C}offea species},
  author = {{S}amson, {N}allapali {P}. and {C}ampa, {C}laudine and {L}e {G}al, {L}{\'e}na{\¨ie}g and {N}oirot, {M}ichel and {T}homas, {G}. and {L}okeswari, {T}.{S}. and {D}e {K}ochko, {A}lexandre},
  editor = {},
  language = {{ENG}},
  abstract = {{H}igh frequency somatic embryogenesis can be obtained over a 7-8 month culture period when using current routing coffee tree micropropagation protocols. {T}o reduce this response time and improve the embryo formation yield, eight different media were tested for primary culture. {T}hese media differed from the classically used ones by their mineral nitrogen and plant growth regulator concentrations. {A}n increase from 0.66 to 0.75 in the {NO}3/{NO}3+{NH}4 ratio and a 2-fold lower plant growth regulator concentration in the primary culture medium led to substantial improvements in terms of rapidity and embryo/plantlet regeneration frequencies. {E}mbryo development time was reduced by up to 3 months with a 5-fold increase in the number of formed embryos. {T}hese results were obtained for the two cultivated coffee tree species, {C}offea canephora and {C}. arabica, and for a wild one, {C}. heterocalyx, but not for a second wild species, {C}. sp. {M}oloundou showing a species-specific response. {T}he new conditions described in this paper led to a substantial enhancement that should be particularly helpful for clonal propagation and genetic engineering of cultivated coffee plants.},
  keywords = {{A}mmonium nitrate ratio ; clonal propagation ; coffee trees ; embryo development ; plant growth regulator},
  booktitle = {},
  journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture},
  volume = {86},
  numero = {1},
  pages = {37--45},
  ISSN = {0167-6857},
  year = {2006},
  DOI = {10.1007/s11240-006-9094-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010035716},
}