@article{fdi:010035640,
  title = {{M}onitoring of the early molecular resistance responses of coffee ({C}offea arabica {L}.) to the rust fungus ({H}emileia vastatrix) using real-time quantitative {RT}-{PCR}},
  author = {{G}anesh, {D}oss and {P}etitot, {A}nne-{S}ophie and {S}ilva, {M}. {C}. and {A}lary, {R}. and {L}ecouls, {A}nne-{C}laire and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{M}olecular resistance responses of coffee ({C}offea arabica {L}.) to the orange rust fungus {H}emileia vastatrix were monitored by real-time quantitative {RT}-{PCR} analysis of gene expression. {S}ignificant activation of coffee genes by fungal infection could be observed around 12-16 h post inoculation (hpi) in the incompatible interaction. {M}icroscopic observations indicated that, at this time, only a limited number of fungal germlings already differentiated a penetration hypha through the stomata. {A}ctivation of the {C}a{WRKY}1 gene, putatively encoding a {WRKY} transcription factor also occurred in the compatible interaction, but was delayed to 24 hpi. {I}n contrast, activation of the {C}a{R}111 gene encoding a protein of unknown function only occurred in the incompatible interaction. {T}he {C}a{NDR}1 gene, an homolog of the {A}rabidopsis non-race specific disease resistance (ndr1) gene was only poorly induced by fungal infection. {W}ounding and salicylic acid treatment markedly activated {C}a{WRKY}1, {C}a{NDR}1 and {C}a{R}111 gene expression. {T}hese results showed that specific transcriptional responses of coffee were detected before penetration of {H}. vastatrix into the leaf had occurred, and suggest a possible role of activated genes in the molecular resistance responses of coffee to the rust fungus. (c) 2005 {E}lsevier {I}reland {L}td. {A}ll rights reserved.},
  keywords = {plant pathogen interactions ; coffee rust ; disease resistance ; {WRKY} transcription factor ; abiotic stresses ; {NDR}1},
  booktitle = {},
  journal = {{P}lant {S}cience},
  volume = {170},
  numero = {6},
  pages = {1045--1051},
  ISSN = {0168-9452},
  year = {2006},
  DOI = {10.1016/j.plantsci.2005.12.009},
  URL = {https://www.documentation.ird.fr/hor/fdi:010035640},
}