@article{fdi:010035634,
  title = {{A}nalysis of the expression pattern conferred by the {P}s{E}nod12{B} promoter from the early nodulin gene of {P}isum sativum in transgenic actinorhizal trees of the {C}asuarinaceae family},
  author = {{S}y, {M}ame {O}ureye and {C}onstans, {L}aure and {O}bertello, {M}ariana and {G}eney, {C}l{\'e}ment and {L}aplaze, {L}aurent and {A}uguy, {F}lorence and {H}ocher, {V}al{\'e}rie and {B}ogusz, {D}idier and {F}ranche, {C}laudine},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o investigate the similarities between nitrogen-fixing symbioses in {R}hizobium-legumes and {F}rankia-actinorhizal plants, we studied the gus expression pattern conferred by the early nodulin promoter {P}s{E}nod12{B} from {P}isum sativum in two transgenic {C}asuarinaceae trees, {A}llocasuarina verticillata and {C}asuarina glauca. {R}espectively 6 and 13 transgenic lines of {C}. glauca and {A}. verticillata were obtained following genetic transformation with the disarmed strain of {A}grobacterium tumefaciens {C}58{C}1 (p{GV}2260; p{BIN}-{P}s{E}nod12{B}-gus). {I}n non-symbiotic tissues, the gus gene was expressed exclusively in the vascular system of the aerial parts of the plants. {I}n mature nodules, {P}s{E}nod12{B} drove some gus expression in large {F}rankia-infected cortical cells. {T}his specificity was different from that observed in pea nodules where {P}s{E}nod12{B} was found expressed in the infection zone. {N}o reporter gene activity was visible at the early stages of the symbiotic process with {F}rankia, including infected root hairs and prenodules. {W}hereas some transcription factors of {A}llocasuarina and {C}asuarina are able to interact with the {P}s{E}nod12{B} promoter, the pattern of expression is modified in nodules of these non-legume heterologous hosts.},
  keywords = {actinorhizal nodule ; {A}llocasuarina verticillata ; {C}asuarina glauca ; {P}s{E}nod12{B} ; beta glucuronidase},
  booktitle = {},
  journal = {{P}lant and {S}oil},
  volume = {281},
  numero = {1-2},
  pages = {281--289},
  ISSN = {0032-079{X}},
  year = {2006},
  DOI = {10.1007/s11104-005-4434-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010035634},
}