%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Calattini, S.
%A Chevalier, S.A.
%A Duprez, R.
%A Bassot, S.
%A Froment, Alain
%A Mahieux, R.
%A Gessain, A.
%T Discovery of a new human T-cell lymphotropic virus (HTLV-3) in Central Africa
%D 2005
%L fdi:010035627
%G ENG
%J Retrovirology
%@ 1742-4690
%M CC:0002368237-0001
%P NIL_1-NIL_4
%R 10.1186/1742-4690-2-30
%U https://www.documentation.ird.fr/hor/fdi:010035627
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-12/010035627.pdf
%V 2
%W Horizon (IRD)
%X Human T-cell Leukemia virus type 1 (HTLV-1) and type 2 (HTLV-2) are pathogenic retroviruses that infect humans and cause severe hematological and neurological diseases. Both viruses have simian counterparts (STLV-1 and STLV-2). STLV-3 belongs to a third group of lymphotropic viruses which infect numerous African monkeys species. Among 240 Cameroonian plasma tested for the presence of HTLV-1 and/or HTLV-2 antibodies, 48 scored positive by immunofluorescence. Among those, 27 had indeterminate western-blot pattern. PCR amplification of pol and tax regions, using HTLV-1, - 2 and STLV-3 highly conserved primers, demonstrated the presence of a new human retrovirus in one DNA sample. tax ( 180 bp) and pol ( 318 bp) phylogenetic analyses demonstrated the strong relationships between the novel human strain (Pyl43) and STLV-3 isolates from Cameroon. The virus, that we tentatively named HTLV-3, originated from a 62 years old Bakola Pygmy living in a remote settlement in the rain forest of Southern Cameroon. The plasma was reactive on MT2 cells but was negative on C19 cells. The HTLV 2.4 western- blot exhibited a strong reactivity to p19 and a faint one to MTA-1. On the INNO-LIA strip, it reacted faintly with the generic p19 (I/II), but strongly to the generic gp46 (I/II) and to the specific HTLV-2 gp46. The molecular relationships between Pyl43 and STLV-3 are thus not paralleled by the serological results, as most of the STLV-3 infected monkeys have an "HTLV-2 like" WB pattern. In the context of the multiple interspecies transmissions which occurred in the past, and led to the present-day distribution of the PTLV-1, it is thus very tempting to speculate that this newly discovered human retrovirus HTLV-3 might be widespread, at least in the African continent.
%$ 020 ; 052