@article{fdi:010035592,
  title = {{U}se of single-point genome signature tags as a universal tagging method for microbial genome surveys},
  author = {{V}an der {L}elie, {D}. and {L}esaulnier, {C}{\'e}line and {M}c{C}orkle, {S}. and {G}eets, {J}. and {T}aghavi, {S}. and {D}unn, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e developed single-point genome signature tags ({SP}-{GST}s), a generally applicable, high-throughput sequencing-based method that targets specific genes to generate identifier tags from well-defined points in a genome. {T}he technique yields identifier tags that can distinguish between closely related bacterial strains and allow for the identification of microbial community members. {SP}-{GST}s are determined by three parameters: (i) the primer designed to recognize a conserved gene sequence, (ii) the anchoring enzyme recognition sequence, and (iii) the type {US} restriction enzyme which defines the tag length. {W}e evaluated the {SP}-{GST} method in silico for bacterial identification using the genes rpo{C}, uvr{B}, and rec{A} and the 16{S} r{RNA} gene. {T}he best distinguishing tags were obtained with the restriction enzyme {C}sp6{I} upstream of the 16{S} r{RNA} gene, which discriminated all organisms in our data set to at least the genus level and most organisms to the species level. {T}he method was successfully used to generate {C}sp6{I}-based tags upstream of the 16{S} r{RNA} gene and allowed us to discriminate between closely related strains of {B}acillus cereus and {B}acillus anthracis. {T}his concept was further used successfully to identify the individual members of a defined microbial community.},
  keywords = {},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {72},
  numero = {3},
  pages = {2092--2101},
  ISSN = {0099-2240},
  year = {2006},
  DOI = {10.1128/{AEM}.72.3.2092-2101.2006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010035592},
}