@article{fdi:010030892,
  title = {{M}olecular identification of the {A}nopheles nili group of {A}frican malaria vectors},
  author = {{K}engne, {P}ierre and {A}wono-{A}mbene, {P}. and {A}ntonio-{N}kondjio, {C}. and {S}imard, {F}r{\'e}d{\'e}ric and {F}ontenille, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{D}istinction between members of the {A}nopheles nili group of mosquitoes ({D}iptera : {C}ulicidae), including major malaria vectors in riverside villages of tropical {A}frica, has been based mainly on doubtful morphological characters. {S}equence variations of the ribosomal {DNA} second internal transcribed spacer ({ITS}2) and {D}3 28{S} region between morphological forms revealed four genetic patterns corresponding to typical {A}n. nili ({T}heobald), {A}n. carnevalei {B}runhes et al., {A}n. somalicus {R}ivola and {H}olstein and the newly identified variant provisionally named {O}veng form. {P}rimers were designed based on {ITS}2 fixed nucleotide differences between haplotypes to develop a multiplex {PCR} for rapid and specific identification of each species or molecular form. {S}pecimens of the {A}n. nili group from {C}ameroon, {B}urkina {F}aso, {I}vory {C}oast and {S}enegal were successfully identified to species, demonstrating the general applicability of this technique based on criteria described in this paper.},
  keywords = {{PALUDISME} ; {VECTEUR} ; {MOUSTIQUE} ; {IDENTIFICATION} ; {BIOLOGIE} {MOLECULAIRE} ; {ANALYSE} {GENETIQUE} ; {TECHNIQUE} {PCR} ; {ANATOMIE} {ANIMALE} ; {BURKINA} {FASO} ; {COTE} {D}'{IVOIRE} ; {CAMEROUN} ; {SENEGAL}},
  booktitle = {},
  journal = {{M}edical and {V}eterinary {E}ntomology},
  volume = {17},
  numero = {1},
  pages = {67--74},
  ISSN = {0269-283{X}},
  year = {2003},
  DOI = {10.1046/j.1365-2915.2003.00411.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010030892},
}