Escoffier S. auteur aut IRD Cayol J.L. auteur aut IRD Ollivier Bernard auteur aut IRD Patel B.K.C. auteur aut IRD Fardeau Marie-Laure auteur aut IRD Thomas P. auteur aut IRD Roger Pierre-Armand auteur aut IRD text journalArticle eng text/pdf reformatted digital access Eighteen strains of anaerobic thiosulfate-reducing bacteria unable to use sulfate as electron acceptor (TSRnSR) were isolated from four ricefield soils originating from France and the Philippines, using peptides as energy sources, H2 as electron donor, thiosulfate as electron acceptors, and four enrichment methods to vary the selection pressure. They were strict proteolytic asaccharolytic anaerobes producing H2S when grown on thiosulfate + H2. They exhibited the same RFLP profile (11 restriction enzymes tested). Partial sequencing of the 16S rDNA showed that they belonged to the genus Clostridium and were phylogenetically related to C. subterminale. DNA-DNA hybridization of a representative strain with the closest C. subterminale strain (DSM 6970T) yielded a value of 68.9%. Previous counts of TSRnSR in ricefield soils, their identification as Clostridium strains, and the known ubiquity of this genus in such soils indicate that TSRnSR may play a significant role in S cycling in some wetland soils. (Résumé d'auteur) specialized BACTERIE MICROFLORE RIZIERE SOL MILIEU DE CULTURE ACTIVITE BIOLOGIQUE SOUFRE REDUCTION SULFATE TECHNIQUE PCR TECHNIQUE RFLP ETUDE EXPERIMENTALE PHYLOGENIE DENDROGRAMME THIOSULFATE FRANCE PHILIPPINES 074SOLMIC 37 145-156 2001 1164-5563 https://www.documentation.ird.fr/hor/fdi:010027113 10.1016/S1164-5563(01)01079-2 1164-5563 [F B010027113] https://www.documentation.ird.fr/hor/fdi:010027113 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/divers2/010027113.pdf IRD - Base Horizon / Pleins textes 2002-01-14 2013-07-31 fdi:010027113 fre