@article{fdi:010027113, title = {{I}dentification of thiosulfate- and sulfur -reducing bacteria unable to reduce sulfate in ricefield soils}, author = {{E}scoffier, {S}. and {C}ayol, {J}.{L}. and {O}llivier, {B}ernard and {P}atel, {B}.{K}.{C}. and {F}ardeau, {M}arie-{L}aure and {T}homas, {P}. and {R}oger, {P}ierre-{A}rmand}, editor = {}, language = {{ENG}}, abstract = {{E}ighteen strains of anaerobic thiosulfate-reducing bacteria unable to use sulfate as electron acceptor ({TSR}n{SR}) were isolated from four ricefield soils originating from {F}rance and the {P}hilippines, using peptides as energy sources, {H}2 as electron donor, thiosulfate as electron acceptors, and four enrichment methods to vary the selection pressure. {T}hey were strict proteolytic asaccharolytic anaerobes producing {H}2{S} when grown on thiosulfate + {H}2. {T}hey exhibited the same {RFLP} profile (11 restriction enzymes tested). {P}artial sequencing of the 16{S} r{DNA} showed that they belonged to the genus {C}lostridium and were phylogenetically related to {C}. subterminale. {DNA}-{DNA} hybridization of a representative strain with the closest {C}. subterminale strain ({DSM} 6970{T}) yielded a value of 68.9%. {P}revious counts of {TSR}n{SR} in ricefield soils, their identification as {C}lostridium strains, and the known ubiquity of this genus in such soils indicate that {TSR}n{SR} may play a significant role in {S} cycling in some wetland soils. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{BACTERIE} ; {MICROFLORE} ; {RIZIERE} ; {SOL} ; {MILIEU} {DE} {CULTURE} ; {ACTIVITE} {BIOLOGIQUE} ; {SOUFRE} ; {REDUCTION} ; {SULFATE} ; {TECHNIQUE} {PCR} ; {TECHNIQUE} {RFLP} ; {ETUDE} {EXPERIMENTALE} ; {PHYLOGENIE} ; {DENDROGRAMME} ; {THIOSULFATE} ; {FRANCE} ; {PHILIPPINES}}, booktitle = {}, journal = {{E}uropean {J}ournal of {S}oil {B}iology}, volume = {37}, numero = {}, pages = {145--156}, ISSN = {1164-5563}, year = {2001}, DOI = {10.1016/{S}1164-5563(01)01079-2}, URL = {https://www.documentation.ird.fr/hor/fdi:010027113}, }