Courgnaud V. auteur aut IRD Pourrut Xavier auteur aut IRD Bibollet-Ruche F. auteur aut IRD Mpoudi Ngolé E. auteur aut IRD Bourgeois A. auteur aut IRD Delaporte Eric auteur aut IRD Peeters Martine auteur aut IRD text journalArticle eng text/pdf reformatted digital access Exploration of the diversity among primate lentiviruses is necessary to elucidate the origins and evolution of immunodeficiency viruses. During a serological survey in Cameroon, we screened 25 wild-born guereza colobus monkeys (Colobus guereza) and identified 7 with HIV/SIV cross-reactive antibodies. In this study, we describe a novel lentivirus, named SIVcol, prevalent in guereza colobus monkeys. Genetic analysis revealed that SIVcol was very distinct from all other known SIV/HIV isolates, with average amino acid identities of 40% for Gag, 50% for Pol, 28% for Env, and around 25% for proteins encoded by five other genes. Phylogenetic analyses confirmed that SIVcol is genetically distinct from other previously characterized primate lentiviruses and clusters independently, forming a novel lineage, the sixth in the current classification. Cercopithecinae monkeys (Old World monkeys) are subdivided into two subfamilies, the Colobinae and the Cercopithecinae, and, so far, all Cercopithecidae monkeys from which lentiviruses have been isolated belong to the Cercopithecinae subfamily. Therefore, SIVcol from guereza colobus monkeys (C. guereza) is the first primate lentivirus identified in the Colobinae subfamily and the divergence of SIVcol may reflect divergence of the host lineage. (Résumé d'auteur) specialized SIDA VIRUS SINGE PHYLOGENIE ANALYSE GENETIQUE TECHNIQUE PCR ETUDE EXPERIMENTALE SIV BIOTAXONOMIE CAMEROUN 052MALTRA03 75 2 857-866 2001 0022-538X https://www.documentation.ird.fr/hor/fdi:010025995 10.1128/JVI.75.2.857-866.2001 0022-538X [F B010025995] https://www.documentation.ird.fr/hor/fdi:010025995 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_59-60/010025995.pdf IRD - Base Horizon / Pleins textes 2001-08-21 2018-08-17 fdi:010025995 fre