@article{fdi:010025985, title = {{C}omparison of direct and membrane feeding methods to infect {A}nopheles arabiensis with {P}lasmodium falciparum}, author = {{A}wono-{A}mbene, {H}.{P}. and {D}iawara, {L}. and {R}obert, {V}incent}, editor = {}, language = {{ENG}}, abstract = {{T}wo standard methods are available to infect mosquitoes with malaria parasites : direct feeding through the skin of the gametocyte carrier, and membrane feeding. #{A}nopheles arabiensis$ collected at larval stages and reared in an insectary were fed in parallel by feeding on #{P}lasmodium falciparum$ gametocyte carriers and by membrane feeding on venous blood of the same gametocyte carriers. {I}nfection of mosquitoes was assessed at {D}ay 7 post bloodmeal by oocyst count of the mosquito midguts. {T}he following parameters were not significantly different between the two methods : the percentage of gametocyte carriers infective for at least one mosquito (52.4% through the skin versus 57.1% through the membrane), the mean infection rate of mosquitoes (10.0% versus 11.3%), the geometric mean oocyst number per mosquito (2.51 versus 3.83). {I}n conclusion, infection of mosquitoes by membrane feeding was similar to infection by direct feeding. {M}ost of the volunteers preferred venipuncture to mosquito bites. ({R}{\'e}sum{\'e} d'auteur)}, keywords = {{PALUDISME} ; {MOUSTIQUE} ; {AGENT} {PATHOGENE} ; {INFECTION} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {ELEVAGE} {DE} {LABORATOIRE} ; {INFESTATION} {EXPERIMENTALE} ; {SENEGAL} ; {THIES}}, booktitle = {}, journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene}, volume = {64}, numero = {1,2}, pages = {32--34}, ISSN = {0002-9637}, year = {2001}, URL = {https://www.documentation.ird.fr/hor/fdi:010025985}, }