@article{fdi:010025074,
  title = {{S}tructure of native and expanded sobemoviruses by electron cryo-microscopy and image reconstruction},
  author = {{O}palka, {N}. and {T}ihova, {M}. and {B}rugidou, {C}hristophe and {K}umar, {A}. and {B}eachy, {R}.{N} and {F}auquet, {C}laude and {Y}eager, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice yellow mottle virus ({RYMV}) and southern bean mosaic virus, cowpea strain ({SCPMV}) are members of the {S}obemovirus genus of {RNA}-containing viruses. {W}e used electron cryo-microscopy (cryo-{EM}) and icosahedral image analysis to examine the native structures of these two viruses at 25 {A} resolution. {B}oth viruses have a single tightly packed capsid layer with 180 subunits assembled on a {T} = 3 icosahedral lattice. {D}istinctive crown-like pentamers emanate from the 12 f-fold axes of symmetry. {T}he exterior face of {SCPMV} displays deep valleys along the 2-fold axes and protrusions at the quasi-3-fold axes. {W}hile having a similar topography, the surface of {RYMV} is comparatively smooth. {T}wo concentric shells of density reside beneath the capsid layer of {RYMV} and {SCPMV}, which we interpret as ordered regions of genomic {RNA}. {I}n the absence of divalent cations, {SCPMV} particles swell and fracture, whereas the expanded form of {RYMV} is stable. {W}e previously proposed that the cell-to-cell movement of {RYMV} in xylem involves chelation of {C}a2+ from pit membranes of infected cells, thereby stabilizing the capsid shells and allowing a pathway for spread of {RYMV} through destabilized membranes. {I}n the context of this model, we propose that the expanded form of {RYMV} is an intermediate in the in vivo assembly of virions. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{RIZ} ; {VIRUS} ; {STRUCTURE} ; {PROTEINE} ; {METHODE} {D}'{ANALYSE} ; {MICROSCOPIE} {ELECTRONIQUE} ; {ANALYSE} {D}'{IMAGE} ; {RAYONNEMENT} {X} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {COTE} {D}'{IVOIRE}},
  booktitle = {},
  journal = {{J}ournal of {M}olecular {B}iology},
  volume = {303},
  numero = {},
  pages = {197--211},
  ISSN = {0022-2836},
  year = {2000},
  DOI = {10.1006/jmbi.2000.4043},
  URL = {https://www.documentation.ird.fr/hor/fdi:010025074},
}