Horizon 1 1 17 ACLN : Articles dans des revues avec comité de lecture non répertoriées par l'AERES Medical and Veterinary Entomology 419-425 POU ELECTROPHORESE ISOENZYME ESTERASE METHODE DE LUTTE INSECTICIDE SENSIBILITE RESISTANCE ETUDE EXPERIMENTALE PHOSPHOGLUCOMUTASE FRANCE MADAGASCAR MALI SENEGAL ETATS UNIS 2000 fdi:010024084 ENG Medical and Veterinary Entomology 0269-283X 4 10.1046/j.1365-2915.2000.00260.x https://www.documentation.ird.fr/hor/fdi:010024084 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_57-58/010024084.pdf 14 Horizon (IRD) Human lice (Phthiraptera : Pediculidae) from Africa, America and Europe were electrophoresed for 28 enzymes, with special interest in metabolic factors likely to be involved with insecticide resistance. Zymogram profiles of the body louse (#Pediculus humanus$ L. from France and U.S.A.) and the head louse (#P. capitis$ DeGeer from France, Madagascar, Mali & Senegal) were compared. Only two enzymes, phosphoglucomutase (Pgm) and esterase 3 (Est-3), showed electrophoretic variation. In our starch gel electrophoresis conditions, #P. humanus$ showed three electromorphs of Pgm migrating anodally 6, 11 and 16 mm (designated alleles a, b, c, respectively). Of the putative Pgm alleles, b and c occured in all samples of both species of lice, whereas allele a was found only in #P. humanus$ lab strain from U.S.A. Esterase 3 had four electromorphs migrating 23, 26, 30 and 35 mm (designated allele a, b, c and d). Among putative Est alleles, a was found only in #P. capitis$ from Bamako (all 14 specimens aa homozygotes), allele d was found only in #P. capitis$ from Dakar (39% frequency), whereas Est-3 alleles b and c showed apparently balanced polymorphism in all samples of both #P. humanus$ and #P. capitis$ except from Bamako. Despite the limited amount of isoenzyme variation detected (only 2/31 polymorphic loci), divergences of Est-3 and Pgm among #Pediculus$ populations may be relevant to their biosystematics and resistance. (Résumé d'auteur) 052TIAC05 ; 052LUTIN03