Peeters Martine auteur aut IRD Esu-Williams E. auteur aut IRD Vergne Laurence auteur aut IRD Montavon Céline auteur aut IRD Mulanga Kabeya Claire auteur aut IRD Harry T. auteur aut IRD Ibironke A. auteur aut IRD Lesage D. auteur aut IRD Patrel Delphine auteur aut IRD Delaporte Eric auteur aut IRD text journalArticle eng text/pdf reformatted digital access The purpose of this study was to generate data on the relative prevalences of the HIV-1 subtypes circulating in Nigeria. A total of 252 HIV-1 positive samples collected during an epidemiologic survey conducted in April 1996 were genetically characterized by HMA (heteroduplex mobility assay) and/or sequencing. Samples were collected in Lagos, Calabar, Kano, and Maiduguri. Overall, the predominant env subtypes were A (61.3%) and G (37.5%). Subtype A is more prevalent in the south (p is less than 0.001), about 70% in Lagos and Calabar, whereas a quarter of the samples was classified as subtype G in these states. In contrast, subtype G is predominant in the north (p is less than 0.001), representing 58% of the samples in Kano. In the northeastern region, Maiduguri, almost similar proportions of subtype A and G were seen, 49 and 47.4%, respectively. A total of 37 samples was also sequenced in the p24 region from the gag gene ; 13 (35%) had discordant subtype designations between env and gag. The majority of the gag (12 of 17) and env (14 of 22) subtype A sequences clustered with the A/G-IBNG strain. Whithin subtype G, three different subclusters were seen among the envelope sequences. These different subclusters are observed among samples obtained from asymptomatic individuals and AIDS patients from the four Nigerian states studied. In conclusion, we observed a limited number of HIV-1 subtypes circulating in Nigeria, with subtypes A and G being the major env subtypes responsible for the HIV-1 epidemic. Nevertheless, the high rate of recombinant viruses (A/G) and the different A/G recombinant structures indicate a complex pattern of HIV-1 viruses circulating in this country. (Résumé d'auteur) specialized SIDA VIRUS PREVALENCE ANALYSE GENETIQUE DIVERSITE GENETIQUE IDENTIFICATION TECHNIQUE PCR REPARTITION GEOGRAPHIQUE NIGERIA KANO 052MALTRA03 16 4 315-325 2000 0889-2229 https://www.documentation.ird.fr/hor/fdi:010024050 10.1089/088922200309197 0889-2229 [F B010024050] https://www.documentation.ird.fr/hor/fdi:010024050 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_57-58/010024050.pdf IRD - Base Horizon / Pleins textes 2001-02-06 2021-05-20 fdi:010024050 fre