@article{fdi:010023750,
  title = {{P}hotosynthetic bradyrhizobia are natural endophytes of the {A}frican wild rice {O}ryza breviligulata},
  author = {{C}haintreuil, {C}l{\'e}mence and {G}iraud, {E}ric and {P}rin, {Y}ves and {L}orquin, {J}ean and {B}â, {A}. and {G}illis, {M}. and {L}ajudie, {P}hilippe de and {D}reyfus, {B}ernard},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e investigated the presence of endophytic rhizobia within the roots of the wetland wild rice #{O}ryza breviligulata$, which is the ancestor of the {A}frican cultivated rice #{O}ryza glaberrima$. {T}his primitive rice species grows in the same wetland sites as #{A}eschynomene sensitiva$, an aquatic stem-nodulated legume associated with photosynthetic strains of #{B}radyrhizobium$. {T}wenty endophytic and aquatic isolates were obtained at three different sites in {W}est {A}frica ({S}enegal and {G}uinea) from nodal roots of #{O}. breviligulata$ and surrounding water by using #{A}. sensitiva$ as a trap legume. {M}ost endophytic and aquatic isolates were photosynthetic and belonged to the same phylogenetic #{B}radyrhizobium$/#{B}lastobacter$ subgroup as the typical photosynthetic #{B}radyrhizobium$ strains previously isolated from #{A}eschynomene$ stem nodules. {N}itrogen-fixing activity, measured by acetylene reduction, was detected in rice plants inoculated with endophytic isolates. {A} 20% increase in the shoot growth and grain yield of #{O}. breviligulata$ grown in a greenhouse was also observed upon inoculation with one endophytic strain and one #{A}eschynomene$ photosynthetic strain. {T}he photosynthetic #{B}radyrhizobium$ sp. {ORS}278 extensively colonized the root surface, followed by intercellular, and rarely intracellular, bacterial invasion of the rice roots, which was determined with a lac{Z}-tagged mutant of {ORS}278. {T}he discovery that photosynthetic #{B}radyrhisobium$ strains, which are usually known to induce nitrogen-fixing nodules on stems of the legume #{A}eschynomene, are also natural true endophytes of the primitve rice #{O}. breviligulata$ could significantly enhance cultivated rice production. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{BACTERIE} ; {ENDOPHYTE} ; {RIZ} ; {RACINE} ; {NODULE} {RACINAIRE} ; {SYMBIOSE} ; {PHOTOSYNTHESE} ; {FIXATION} {BIOLOGIQUE} {DE} {L}'{AZOTE} ; {INOCULATION} ; {CULTURE} {DE} {MICROORGANISME} ; {ETUDE} {EXPERIMENTALE} ; {SENEGAL} ; {GUINEE}},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {66},
  numero = {12},
  pages = {5437--5447},
  ISSN = {0099-2240},
  year = {2000},
  DOI = {10.1128/{AEM}.66.12.5437-5447.2000},
  URL = {https://www.documentation.ird.fr/hor/fdi:010023750},
}