@article{fdi:010023352,
  title = {{D}iagnosis of human trypanosomiasis, due to {T}rypanosoma brucei gambiense in central {A}frica, by the polymerase chain reaction},
  author = {{P}enchenier, {L}aurent and {S}imo, {G}. and {G}r{\'e}baut, {P}ascal and {N}kinin, {S}. and {L}aveissi{\`e}re, {C}laude and {H}erder, {S}t{\'e}phane},
  editor = {},
  language = {{ENG}},
  abstract = {{D}uring a mass screening of sleeping sickness conducted in 1998 and 1999, and involving 27,932 persons in {C}ameroon and the {C}entral {A}frican {R}epublic, we tested the polymerase chain reaction ({PCR}) on whole blood for the diagnosis of human {A}frican trypanosomiasis due to #{T}rypanosoma brucei gambiense$. {T}he 1858 samples obtained were from 4 groups : 155 infected patients, 1432 serological suspects detected by the card agglutination test for trypanosomiasis ({CATT}), 222 negative controls living in the prospected area (negative methods) and unexposed to the disease ({E}uropeans). {T}he technique of {DNA} extraction used made it possible to preserve the blood samples in the field. {T}he primers used were specific for #{T}. brucei$ s.l. {O}nly 1 patient was {PCR} negative, and 3 of the negative controls, exposed to the disease, were {PCR} positive. {A}mong the 1432 serological suspects, only 50 were {PCR} positive. {D}uring the 6-month follow-up after the surveys, the {PCR} results are unlikely to have been due to a cross-reaction with #{T}. brucei brucei$, which is non-pathogenic for man, but are more likely to have resulted from a mislabelling of sample tubes. {A}ll control indiviudals, exposed or not to the disease, were negative by {PCR}. {T}he {PCR}-negative patient was possibly a registration error. {A}mong 50 {PCR} positive serological suspects, 39 of them were re-examined. {F}ive were found to be positive by the kit for in-vitro isolation of trypanosomes, representing an increase in patients of almost 13%. {A}t the end of the study, 160 patients were diagnosed, and the {PCR} was positive for 159 of them (99.4%). {M}oreover, the {PCR} made it possible to reduce the number of suspects to be re-examined (50 instead of 1432 ; a reduction of 96.5%). ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{TRYPANOSOMIASE} {HUMAINE} ; {MALADIE} {DU} {SOMMEIL} ; {DIAGNOSTIC} ; {TECHNIQUE} {PCR} ; {ETUDE} {COMPARATIVE} ; {AFRIQUE} {CENTRALE} ; {CAMEROUN} ; {YAOUNDE} ; {FONTEM} ; {CAMPO} ; {BIPINDI} ; {BATANGAFO}},
  booktitle = {},
  journal = {{T}ransactions of the {R}oyal {S}ociety of {T}ropical {M}edicine and {H}ygiene},
  volume = {94},
  numero = {4},
  pages = {392--394},
  ISSN = {0035-9203},
  year = {2000},
  DOI = {10.1016/{S}0035-9203(00)90116-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010023352},
}