%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Bonnet, Sarah
%A Gouagna, C.
%A Safeukui, I.
%A Meunier, Jean-Yves
%A Boudin, Christian
%T Comparison of artificial membrane feeding with direct skin feeding to estimate infectiousness of Plasmodium falciparum gametocyte carriers to mosquitoes
%D 2000
%L fdi:010021775
%G ENG
%J Transactions of the Royal Society of Tropical Medicine and Hygiene
%@ 0035-9203
%K PALUDISME ; AGENT PATHOGENE ; TRANSMISSION ; ELEVAGE DE LABORATOIRE ; EVALUATION
%K REPAS DE SANG ; TRANSMISSION HOMME/VECTEURL ; INFESTATION EXPERIMENTALE ; REPAS DIRECT ; REPAS SUR MEMBRANE
%K CAMEROUN ; YAOUNDE
%M ISI:000085825800033
%N 1
%P 103-106
%R 10.1016/S0035-9203(00)90456-5
%U https://www.documentation.ird.fr/hor/fdi:010021775
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_55-56/010021775.pdf
%V 94
%W Horizon (IRD)
%X Human infectiousness to mosquitoes can be estimated by 2 tests : direct feeding on the skin and membrane feeding on venous blood. To validate the membrane feeding assay, the infectiousness of #Plasmodium falciparum$ gametocyte carriers to #Anopheles gambiae$ was estimated by these 2 methods in the same individuals in a rural area of Cameroon. Results from 37 experiments showed that direct feeding gave significantly higher infection rates than membrane feeding. We observed an average of 19.4% infected mosquitoes by direct feeding compared with 12.1% by membrane feeding, and a mean oocyst load of 5.63 by direct feeding compared with 2.65 by membrane feeding. However, there was a very good concordance between the 2 tests : 84.3% with the Kappa test on percentages of infected mosquitoes and 98.7% with the interclass correlation coefficient on oocyst loads. In addition, we found a good linear correlation between the 2 methods. (Résumé d'auteur)
%$ 052ANOPAL03