@article{fdi:010020868,
  title = {{B}eneficial effect of post-thawing osmoconditioning on the recovery of cryopreserved coffee ({C}offea arabica {L}.) seeds},
  author = {{D}ussert, {S}t{\'e}phane and {C}habrillange, {N}athalie and {V}asquez, {N}. and {E}ngelmann, {F}lorent and {A}nthony, {F}ran{\c{c}}ois and {G}uyot, {A}. and {H}amon, {S}erge},
  editor = {},
  language = {{ENG}},
  abstract = {{O}smoconditioning - controlled rehydration of seeds in a solution with low osmotic potential - has been shown to reinvigorate aged seeds. {T}he present work aimed at investigating the effect of osmoconditioning on the germination of cryopreserved seeds of {C}offea arabica, whose viability and vigour are drastically affected by cryopreservation. {F}or cryopreservation, seeds were desiccated to 0.21 g {H}2{O}/g dw, cooled at 1°{C}/min to -50°{C}, then immersed rapidly in liquid nitrogen. {A}fter rapid rewarming, seeds were osmoconditioned for 1 to 6 weeks using solutions with osmotic potentials between -1 and -4 {MP}a. {T}he time to produce half of the final percentage of normal seedlings, {T}50, was about three fold lower with osmoconditioned seeds than with non-osmoconditioned seeds (12-14 d vs 36 d). {M}oreover, after a 6-week osmoconditioning treatment with solutions with osmotic potential of -1 and -1.25 {MP}a, the percentage of seedlings recovered from cryopreserved seeds was 64-74%, against 13-16% only for cryopreserved seeds which were not osmoconditioned. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{SEMENCE} ; {CAFE} ; {AMELIORATION} {DES} {PLANTES} ; {CONSERVATION} {DES} {RESSOURCES} {GENETIQUES} ; {DESSICCATION} ; {REHYDRATATION} ; {GERMINATION} ; {POTENTIEL} {OSMOTIQUE} ; {CRYOCONSERVATION}},
  booktitle = {},
  journal = {{C}ryo-{L}etters},
  volume = {21},
  numero = {1},
  pages = {47--52},
  ISSN = {0143-2044},
  year = {2000},
  URL = {https://www.documentation.ird.fr/hor/fdi:010020868},
}