@article{fdi:010020690,
  title = {{M}olecular analysis of introgressive breeding in coffee ({C}offea arabica {L}.)},
  author = {{L}ashermes, {P}hilippe and {A}ndrzejewski, {S}andra and {B}ertrand, {B}. and {C}ombes, {M}arie-{C}hristine and {D}ussert, {S}t{\'e}phane and {G}raziosi, {G}. and {T}rouslot, {P}ierre and {A}nthony, {F}ran{\c{c}}ois},
  editor = {},
  language = {{ENG}},
  abstract = {{N}ineteen arabica coffee introgression lines ({BC}1{F}4) and two accessions derived from a spontaneous interspecific cross (i.e. {T}imor {H}ybrid) between #{C}offea arabica$ (2n=4x=44) and #{C}. canephora$ (2n=2x=22) were analysed for the introgression of #{C}. canephora$ genetic material. {T}he {T}imor {H}ybrid-derived genotypes were evaluated by {AFLP}, using 42 different primer combinations, and compared to 23 accessions of #{C}. arabica$ and 8 accessions of #{C}. canephora$. {A} total of 1062 polymorphic fragments were scored among the 52 accessions analysed. {O}ne hundred and seventy-eight markers consisting of 109 additional bands (i.e. introgressed markers) and 69 missing bands distinguished the group composed of the {T}imor {H}ybrid-derived genotypes from the accessions of #{C}. arabica$. {AFLP} therefore seemed to be an extremely efficient technique for {DNA} marker generation in coffee as well as for the detection of introgression in #{C}. arabica$. {T}he genetic diversity observed in the {T}imor {H}ybrid-derived genotypes appeared to be approximately double that in #{C}. arabica$. {A}lthough representing only a small proportion of the genetic diversity available in #{C}. canephora$, the {T}imor {H}ybrid obviously constitutes a considerable source of genetic diversity for arabica breeding. {A}nalysis of genetic relationships among the {T}imor {H}ybrid-derived genotypes suggested that introgression was not restricted to chromosome substitution but also involved chromosome recombinations. {F}urthermore, the {T}imor {H}ybrid-derived genotypes varied considerably in the number of {AFLP} markers attributable to introgression. {I}n this way, the introgressed markers identified in the analysed arabica coffee introgressed gentotypes were estimated to represent from 9% to 29% of the #{C}. canephora$ genome. {N}evertheless, the amount of alien genetic material in the introgression arabica lines remains substantial and should justify the development of adapted breeding strategies. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{HYBRIDATION} {INTERSPECIFIQUE} ; {HYBRIDE} ; {AMELIORATION} {DES} {PLANTES} ; {AMELIORATION} {GENETIQUE} ; {DIVERSITE} {GENETIQUE} ; {GENOTYPE} ; {MARQUEUR} {GENETIQUE} ; {ANALYSE} {GENETIQUE} ; {ETUDE} {EXPERIMENTALE} ; {INTROGRESSION} ; {TECHNIQUE} {AFLP}.{AMPLIFIED} {FRAGMENT} {LENGTH} {POLYMORPHISM}},
  booktitle = {},
  journal = {{T}heoretical and {A}pplied {G}enetics},
  volume = {100},
  numero = {1},
  pages = {139--146},
  ISSN = {0040-5752},
  year = {2000},
  DOI = {10.1007/s001220050019},
  URL = {https://www.documentation.ird.fr/hor/fdi:010020690},
}