@inproceedings{fdi:010020351,
  title = {{E}ffects of varying latency period on the in vivo survival of ova after {O}vaprim- and h{CG}-induced ovulationin the {A}sian catfish {P}angasius hypophthalmus ({S}iluriformes, {P}angasiidae)},
  author = {{L}egendre, {M}arc and {S}lembrouck, {J}acques and {S}ubagja, {J}. and {K}ristanto, {A}.{H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}ver a 2-year period at the {S}ukamandi station ({W}est {J}ava, {I}ndonesia), 87 #{P}. hypophthalmus$ females selected on the basis of a modal oocyte diameter greater than 1.0 mm were treated with {O}vaprim (n=77) or h{CG} (n=10) to induce oocyte maturation and ovulation. {T}he two hormonal treatment led to similar results in terms of ovulation percentage (86 and 90%), hatching rates (69 plus or minus 28 and 82 plus or minus 11%) and relative fecundity (167,000 plus or minus 18,000 and 128,000 plus or minus 60,000 eggs/kg, with {O}vaprim and h{CG} respectively. {T}he latency period between the last hormone injection and ovulation was negatively correlated to water temperature but showed important variations at a same temperature depending on individual females (e.g. between 5-11 h at 28-29°{C}). {T}he ovulation time was therefore difficult to predict accurately in this species. {T}he assessment of survival time of ova maintained in ovario after ovulation showed that the process of ageing (overripening of ova) occurs rapidly in #{P}. hypophthalmus$. {T}he overall quality of ova begun to decrease as early as 2 hours after ovulation and, after 3 hours, hatching rate dropped down and fraction of deformed larvae increased significantly in comparison to those observed at the moment of ovulation. {I}n some individual females this evolution was even more rapid, with a sharp decrease in hatching rates between 1 and 2 hours post-ovulation. {T}he duration of ova survival did not appear to depend on the type of hormonal treatment used ({O}vaprim or h{CG}). {F}or optimised gamete management in hatcheries, it is therefore recommended to check carefully the females for the occurrence of ovulation (between 3 to 11 h after the last hormone injection, depending on water temperature) and to collect and fertilise the eggs within less than two hours after this moment. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{POISSON} {D}'{EAU} {DOUCE} ; {PISCICULTURE} ; {METHODE} {D}'{ELEVAGE} ; {REPRODUCTION} ; {INDUCTION} ; {HORMONE} ; {OVOCYTE} ; {OEUF} ; {LARVE} ; {TAUX} {DE} {SURVIE} ; {ECLOSION} ; {FECONDATION} ; {ETUDE} {EXPERIMENTALE} ; {REPRODUCTION} {ARTIFICIELLE} ; {TRAITEMENT} {HORMONAL} ; {GNRH} ; {DOMPERIDONE} ; {OVAPRIM} ; {HCG}.{HUMAN} {CHRONIC} {GONADOTROPINE} ; {INDONESIE} ; {JAWA} ; {SUKAMANDI} {STATION}},
  numero = {},
  pages = {119--125},
  booktitle = {{T}he biological diversity and aquaculture of clariid and pangasiid catfishes in {S}outh-{E}ast {A}sia : proceedings of the mid-term workshop of the "{C}atfish {A}sia project"},
  year = {1998},
  URL = {https://www.documentation.ird.fr/hor/fdi:010020351},
}